植物提取物处理马铃薯黑腿病的体外评价

N. Liaqat
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引用次数: 0

摘要

马铃薯黑腿病是由萎败乳杆菌引起的一种严重病害。为分离和鉴定病原菌,采集病样,通过科赫假设、革兰氏染色、KOH试验和马铃薯块茎试验进行处理和确认。采用完全随机设计(CRD)的抑菌区技术,对9种植物提取物P. guajava(番石榴)、P. granatum(石榴)、S. pinnata(李子)、S. cumini (Kalajam)、M. indica(芒果)、T. indica、L.chinensis(荔枝)、V.amurensis(葡萄)和C. verum(肉桂)的抗Pa活性进行了评价。最大抑菌区分别为黄棘棘菌(7.640 mm? ?)、绿棘棘菌(6.566 mm? ?)、葡萄棘菌(4.159 mm? ?)、籼稻棘菌(3.437 mm? ?)、石榴棘菌(2.918 mm? ?)、番石榴棘菌(2.844 mm? ?)、皮棘菌(2.751 mm? ?)、籼稻棘菌(2.344 mm? ?)、中华棘菌(2.251 mm? ?)处理与浓度间的交互作用表明,C1浓度(6.955)表达的最大抑制区为verunn(5.177)、vinifera(3.177)、indica(3.288)、p.a granatum(1.844)、p.a guajava(2.066)、S. piñata(2.288)、m.a indica(1.066)、l.c chinensis (1.733) mm, C2浓度(7.455)表达的最大抑制区为verunn(7.177)、c.a vinifera(4.122)、T. indica(3.344)、p.a granatum(3.344)、l.a granatum(3.344)。与对照相比,在C3浓度下瓜石榴(2.677)、piñata(3.344)、印度栗(2.733)、中华栗(2.344)mm的抑制区分别为8.511、7.344、5.177、3.677、3.566、3.788、3.400、3.566、2.677mm。处理间和时间间的互作结果表明,与对照相比,柠条(6.844、7.622、8.455)对H1、H2和H3的抑制区最大,羊草(1.566、2.233、2.955)对H3的抑制区最小。
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IN-VITRO EVALUATION OF PLANT EXTRACTS FOR THE MANAGEMENT OF BLACK LEG OF POTATO
Potato crop is facing serious threat due to blackleg disease caused by Pectobacterium atrosepticum. For pathogen isolation and identification, diseased samples were collected, processed and confirmed through Koch’s postulates, gram staining, KOH test and Potato tuber assay. Nine plants extracts P. guajava (Guava), P. granatum (Pomegranate), S. pinnata (Plum), S. cumini (Kalajam), M. indica (Mango), T. indica, L.chinensis (Litchi), V.amurensis (Grapes) and C. verum (Cinnamon) were evaluated against Pa by using inhibition zone technique under Completely Randomized Design (CRD). Maximum inhibition zone was measured by S. cumini (7.640 mm?) followed by C. verunn (6.566), C. vinifera (4.159), T. indica (3.437), P. granatum (2.918), p.guajava (2.844), S. pinata (2.751), M. indica (2.344), L. chinensis (2.251) as compared to control, respectively. Interaction between treatments and concentrations indicated that C1 concentration of S. cumini (6.955) expressed maximum inhibition zone as compared to C. verunn (5.177), C. vinifera (3.177), T. indica (3.288), P. granatum (1.844), p.guajava (2.066), S. piñata (2.288), M. indica (1.066), L. chinensis (1.733) mm while C2 concentration of S. cumini (7.455) also expressed maximum inhibition zone followed by C. verunn (7.177), C. vinifera (4.122), T. indica (3.344), P. granatum (3.344), p.guajava (2.677), S. piñata (3.344), M. indica (2.733), L. chinensis (2.344) mm and at C3 concentration exhibited 8.511,7.344, 5.177, 3.677, 3.566, 3.788, 3.400, 3.566, 2.677mm inhibition zone of treatments as compared to control respectively. While interaction between treatments and hours showed that S. cumini (6.844, 7.622, 8.455) exhibited maximum inhibition zone and L. chinensis (1.566, 2.233, 2.955) expressed minimum inhibition zone at H1, H2 and H3 as compared to control respectively.
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