动物战利品在博物馆保护实践中的作用:喀麦隆西部地区宫殿博物馆的案例研究

GABJ Pub Date : 2020-01-25 DOI:10.46325/gabj.v5i1.162
P.M.M Mfonden, F. Meutchieye
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引用次数: 0

摘要

故宫博物院藏有重要的动物遗骸(战利品),喀麦隆西部地区的故宫博物院也是如此。本文从遗传保护的角度对故宫博物院的动物馆藏进行了初步研究。我们调查了喀麦隆西部地区的11个酋长宫殿博物馆。我们收集了这些奖杯的样本。我们使用dnasy®血液和组织试剂盒从这些奖杯中提取和纯化DNA。采用琼脂糖凝胶电泳和分光光度法进行定量和质量控制。琼脂糖凝胶电泳显示,64%的标本具有可能质量良好的DNA, 12%的标本具有受污染的DNA, 12%的标本具有降解的DNA。分光光度法测定显示,28%的标本具有可量化的DNA,而72%的标本DNA含量较差。具有高质量DNA的标本可用于进一步研究(例如,用于野生动物可追溯性的DNA测序)。为了通过博物馆使传统所发挥的保护作用永久化,行政当局必须在实际正在修订的森林法中对传统和文化活动中战利品的获取和使用进行管制。博物馆必须确保符合获取和管理野生动物战利品的标准
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Roles of animal trophies in museums on conservation practices: case study of Palace museums of the west region of Cameroon
Palace museums harbour important animal remains (trophies), this is the case too with palace museums of the West region of Cameroon. Here we present a preliminary study into the animal-based collections of palace museums from a genetic conservation perspective. We surveyed 11 chiefdom palace museums in the West Region of Cameroon. We collected samples of these trophies. We extracted and purified DNA from these trophies using the DNeasy® Blood and Tissue Kit. Quantification and quality control were done using the agarose gel electrophoresis and spectrophotometric methods. From agarose gel electrophoresis, 64% of specimens have DNA that could be of good quality, 12% of specimens have contaminated DNA, while 12% of specimens have degraded DNA. Spectrophotometric determination reveals that 28% of the specimens has quantifiable DNA against 72% that was of poor DNA quantity. Specimens with good quality DNA could be used for further studies (for instance, DNA sequencing for wildlife traceability). In order to perpetuate the conservation role played by the tradition through museums, the administration has to regulate the acquisition and use of trophies in traditional and cultural events in the forest law actually under revision. Museums have to ensure compliance with the criteria of acquisition and management of wildlife trophies
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