木霉对那加兰邦鸽豆不同病害的体外鉴定

Betikundang Chang, N. Ao, Kavi Sumi, Narola Pongener, P. Neog
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摘要

本研究于2020年8月至10月在印度那加兰邦大学(NU) Medziphema校区植物病理系植物病理系实验室进行,目的是评价木霉(harzianum, asperellum和T. virens)对3种木豌豆病害的拮抗潜力,即:互花叶斑病(Alternaria alternata)、颈腐病(Sclerotium rolfsii)和疫霉(Phytophthora drechsleri f. sp. cajani)。采用双培养技术对这些拮抗剂作为生物制剂对病原菌的拮抗效果进行了研究。体外筛选试验结果表明,所有生物制剂均能显著抑制病原菌菌丝的生长。3种木霉中,哈氏霉(55.53%)对互生木霉的抑制率最高,曲霉(50.87%)对罗氏木霉的抑制率最高,而绿霉(55.88%)对木霉的抑制率最高。因此,体外研究表明木霉属具有作为生物制剂用于防治鸽豌豆褐斑病、颈腐病和疫霉病的潜力。
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In-vitro evaluations of Trichoderma spp. against Different Diseases of Pigeonpea in Nagaland
The investigation was carried out at the laboratory of Plant Pathology Department, SASRD, Nagaland University (NU), Medziphema Campus, Nagaland, India during the year kharif (August-October, 2020) to evaluate the antagonistic potentiality of Trichoderma spp. (T. harzianum, T. asperellum and T. virens) against three diseases of pigeonpea viz. Alternaria leaf spot (Alternaria alternata), Collar rot (Sclerotium rolfsii), Phytophthora blight (Phytophthora drechsleri f. sp. cajani). Dual culture technique was followed to test the efficacy of these antagonists as bio-agents against the pathogens. Result of in vitro screening test revealed that all the bio-agents significantly inhibited the mycelial growth of the pathogens. Among the three Trichoderma spp. evaluated, T. harzianum (55.53%) was found to be the highest in per cent inhibition of A. alternata, T. asperellum (50.87 %) recorded highest per cent inhibition on S. rolfsii and T. virens (55.88%) recorded highest per cent inhibition on Phytophthora drechsleri f. sp. cajani. Thus, the in vitro study suggests that the Trichoderma spp. has a potential to be used as bio-agents in managing these three fungal diseases viz. Alternaria leaf spot, Collar rot and Phytophthora blight of pigeonpea.
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