Autoproteolytic Processing and Processing-dependent Secretion of Aspergillus oryzae Alkaline Protease in Yeast

visiae, directed the secretion of enzymatically active Alp into culture medium with its N-terminus identical to that of native Alp, demonstrating that the prepro region of 121 amino acids was processed correctly in the yeast.2) The deduced pre region of around 20 amino acids at the N-terminus of the prepro region is thought to be eliminated through translocation across the endoplasmic reticulum lumen by a signal peptidase, but it is not certain how the remaining pro region is processed. Two alternative mechanisms, autoproteolysis and the involvement of a processing enzyme, can be considered in the processing of proteases. With regard to the processing