{"title":"酵母细胞不对称还原苯甲酰甲酸甲酯合成(R)-(-)-扁桃酸甲酯","authors":"Ou Zhimin, Liu Yong, Nan Yinkang","doi":"10.1109/ICBEB.2012.390","DOIUrl":null,"url":null,"abstract":"An efficient yeast cell biotransformation process was set up for asymmetric synthesis of (R)-(-)-mandelic acid methyl ester, a key drug intermediate. Saccharomyces cerevisiae 21 was selected as optimum strain for biotransformation. The optimum reduction conditions are as follows: substrate concentration 22 g/L, cell concentration 150 g/L, reaction time 36 h, 30°C, pH5.0. Conversion and enantiometric excess of (R)-(-)-mandelic acid methyl ester reached 99.4% and 99.9%.","PeriodicalId":6374,"journal":{"name":"2012 International Conference on Biomedical Engineering and Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2012-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Synthesis of (R)-(-)-Mandelic Acid Methyl Ester by Asymmetric Reduction of Methyl Benzoylformate with Yeast Cells\",\"authors\":\"Ou Zhimin, Liu Yong, Nan Yinkang\",\"doi\":\"10.1109/ICBEB.2012.390\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"An efficient yeast cell biotransformation process was set up for asymmetric synthesis of (R)-(-)-mandelic acid methyl ester, a key drug intermediate. Saccharomyces cerevisiae 21 was selected as optimum strain for biotransformation. The optimum reduction conditions are as follows: substrate concentration 22 g/L, cell concentration 150 g/L, reaction time 36 h, 30°C, pH5.0. Conversion and enantiometric excess of (R)-(-)-mandelic acid methyl ester reached 99.4% and 99.9%.\",\"PeriodicalId\":6374,\"journal\":{\"name\":\"2012 International Conference on Biomedical Engineering and Biotechnology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2012-05-28\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"2012 International Conference on Biomedical Engineering and Biotechnology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1109/ICBEB.2012.390\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"2012 International Conference on Biomedical Engineering and Biotechnology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1109/ICBEB.2012.390","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Synthesis of (R)-(-)-Mandelic Acid Methyl Ester by Asymmetric Reduction of Methyl Benzoylformate with Yeast Cells
An efficient yeast cell biotransformation process was set up for asymmetric synthesis of (R)-(-)-mandelic acid methyl ester, a key drug intermediate. Saccharomyces cerevisiae 21 was selected as optimum strain for biotransformation. The optimum reduction conditions are as follows: substrate concentration 22 g/L, cell concentration 150 g/L, reaction time 36 h, 30°C, pH5.0. Conversion and enantiometric excess of (R)-(-)-mandelic acid methyl ester reached 99.4% and 99.9%.
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