采用常规巢式PCR测定母体血清早期胎儿性别:新技术及其在纳西里耶省的临床应用。

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引用次数: 1

摘要

这项工作是在伊拉克蒂卡尔大学的癌症研究中心进行的。本研究表明,一个可靠的,非侵入性的性别确定可以实现PCR分析母体血清在怀孕的前三个月。与影响母亲和胎儿的侵入性方法的风险相反,应用无细胞胎儿DNA (cffDNA)被证明是非常有效的,风险更低。产前诊断的应用之一是胎儿性别确定,这对有性别相关遗传病风险的胎儿很重要。在这种情况下,通过获得性别的基本信息,可以在治疗中进行必要的时间管理,大大减少了使用侵入性方法的必要性。本研究的目的是利用x - 5染色体长臂上FMR1基因的ATL - 1位点和y染色体特异性序列的多重PCR扩增方法,对妊娠早期胎儿性别进行简单的多重PCR检测。本研究共纳入52例孕妇,52例血清标本均检测到ATL1特异性序列,其中23例血清标本产生y染色体特异性片段。29份血清样本均未检出y特异性片段。
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Early fetal gender determination using convention nested PCR assay of maternal serum: New technologies and clinical applications in Nasiriya province.
This work was carried out in the cancer research Thi-Qar University, Iraq. This study demonstrates that a reliable, non-invasive sex determination can be achieved by PCR analysis of maternal serum during the first trimester of pregnancy. In contrary to the risks of invasive methods that affect both mother and fetus, applying cell-free fetal DNA (cffDNA) is proven highly effective with lower risk. One of the applications of prenatal diagnosis is fetal gender determination, which is important in fetuses at risk of sex-linked genetic diseases. In such cases by obtaining the basic information of the gender, necessary time management can be taken in therapeutic to significantly reduce the necessity of applying the invasive methods. The aim of this study was to use simple multiplex PCR protocol of fetal gender at early stage of gestation applying a multiplex PCR amplification of the ATL 1 locus in the FMR1 gene located on the long arm of X-chromosome5 and the Y-chromosome-specific sequence. A total of 52 pregnant women participated in this study, The ATL1 specific sequences were detected in all 52 serum specimens, while the Y-chromosome-specific fragment were generated in 23 of serum samples. The Y-specific fragment was not detected in any of the 29 serum samples of serum.
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