{"title":"采用常规巢式PCR测定母体血清早期胎儿性别:新技术及其在纳西里耶省的临床应用。","authors":"","doi":"10.32792/utq/utj/vol13/3/7","DOIUrl":null,"url":null,"abstract":"This work was carried out in the cancer research Thi-Qar University, Iraq. This study demonstrates that a reliable, non-invasive sex determination can be achieved by PCR analysis of maternal serum during the first trimester of pregnancy. In contrary to the risks of invasive methods that affect both mother and fetus, applying cell-free fetal DNA (cffDNA) is proven highly effective with lower risk. One of the applications of prenatal diagnosis is fetal gender determination, which is important in fetuses at risk of sex-linked genetic diseases. In such cases by obtaining the basic information of the gender, necessary time management can be taken in therapeutic to significantly reduce the necessity of applying the invasive methods. The aim of this study was to use simple multiplex PCR protocol of fetal gender at early stage of gestation applying a multiplex PCR amplification of the ATL 1 locus in the FMR1 gene located on the long arm of X-chromosome5 and the Y-chromosome-specific sequence. A total of 52 pregnant women participated in this study, The ATL1 specific sequences were detected in all 52 serum specimens, while the Y-chromosome-specific fragment were generated in 23 of serum samples. The Y-specific fragment was not detected in any of the 29 serum samples of serum.","PeriodicalId":23465,"journal":{"name":"University of Thi-Qar Journal","volume":"11 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2019-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":"{\"title\":\"Early fetal gender determination using convention nested PCR assay of maternal serum: New technologies and clinical applications in Nasiriya province.\",\"authors\":\"\",\"doi\":\"10.32792/utq/utj/vol13/3/7\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"This work was carried out in the cancer research Thi-Qar University, Iraq. This study demonstrates that a reliable, non-invasive sex determination can be achieved by PCR analysis of maternal serum during the first trimester of pregnancy. In contrary to the risks of invasive methods that affect both mother and fetus, applying cell-free fetal DNA (cffDNA) is proven highly effective with lower risk. One of the applications of prenatal diagnosis is fetal gender determination, which is important in fetuses at risk of sex-linked genetic diseases. In such cases by obtaining the basic information of the gender, necessary time management can be taken in therapeutic to significantly reduce the necessity of applying the invasive methods. The aim of this study was to use simple multiplex PCR protocol of fetal gender at early stage of gestation applying a multiplex PCR amplification of the ATL 1 locus in the FMR1 gene located on the long arm of X-chromosome5 and the Y-chromosome-specific sequence. A total of 52 pregnant women participated in this study, The ATL1 specific sequences were detected in all 52 serum specimens, while the Y-chromosome-specific fragment were generated in 23 of serum samples. The Y-specific fragment was not detected in any of the 29 serum samples of serum.\",\"PeriodicalId\":23465,\"journal\":{\"name\":\"University of Thi-Qar Journal\",\"volume\":\"11 1\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2019-04-25\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"University of Thi-Qar Journal\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.32792/utq/utj/vol13/3/7\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"University of Thi-Qar Journal","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.32792/utq/utj/vol13/3/7","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Early fetal gender determination using convention nested PCR assay of maternal serum: New technologies and clinical applications in Nasiriya province.
This work was carried out in the cancer research Thi-Qar University, Iraq. This study demonstrates that a reliable, non-invasive sex determination can be achieved by PCR analysis of maternal serum during the first trimester of pregnancy. In contrary to the risks of invasive methods that affect both mother and fetus, applying cell-free fetal DNA (cffDNA) is proven highly effective with lower risk. One of the applications of prenatal diagnosis is fetal gender determination, which is important in fetuses at risk of sex-linked genetic diseases. In such cases by obtaining the basic information of the gender, necessary time management can be taken in therapeutic to significantly reduce the necessity of applying the invasive methods. The aim of this study was to use simple multiplex PCR protocol of fetal gender at early stage of gestation applying a multiplex PCR amplification of the ATL 1 locus in the FMR1 gene located on the long arm of X-chromosome5 and the Y-chromosome-specific sequence. A total of 52 pregnant women participated in this study, The ATL1 specific sequences were detected in all 52 serum specimens, while the Y-chromosome-specific fragment were generated in 23 of serum samples. The Y-specific fragment was not detected in any of the 29 serum samples of serum.