Xanthene-stained nanoparticles for phosphorescence anisotropy measurements

Abstract For the measurement of flow-induced microrotations in flows utilizing the depolarization of phosphorescence anisotropy, suitable luminophores are crucial. The present work examines dyes of the xanthene family, namely Rhodamine B, Eosin Y and Erythrosine B. Both in solution and incorporated in particles, the dyes are examined regarding their luminescent lifetimes and their quantum yield. In an oxygen-rich environment at room temperature, all dyes exhibit lifetimes in the sub-microsecond range and a low intensity signal, making them suitable for sensing fast rotations with sensitive acquisition systems.