V. Shivakumar, A. Tegginamani, N. Zain, Avita Rath, A. Zamzuri
{"title":"不同形式茶提取物(Camellia sinensis)对龋病病原菌的抑菌效果","authors":"V. Shivakumar, A. Tegginamani, N. Zain, Avita Rath, A. Zamzuri","doi":"10.13005/bpj/2678","DOIUrl":null,"url":null,"abstract":"Introduction: Dental caries is a frequent chronic infectious condition in the general population. Streptococcus mutans and Lactobacillus acidophilus play a significant part in the cause of dental caries. Various antimicrobials have been tried to prevent these microorganisms. Traditional herbal medicine extracted from plants has been employed as a remedy. Among them, tea leaf extract showed promising antimicrobial properties against infections. Aims: The current study was designed to assess the antibacterial activity of various types of tea extract against cariogenic microorganisms. Methods: S. mutans and L. acidophilus were grown in this in-vitro experimental study and maintained in their respective agars. Three different concentrations of 250 mg/ml aqueous, 250 mg/ml ethanolic, and 250 mg/ml aqueous with sugar solutions were prepared from Japanese green tea, Oolong Chinese tea, and Sabah black tea. Kirby-Bauer disc diffusion method was used to assess the antibacterial activity of tea extracts. As positive and negative control groups, 0.2% chlorhexidine and 1% DMSO were employed. The zone of inhibition was determined in millimetres following a 24-hour incubation period at 37 °C. Results: Japanese green tea at its three different concentrations exhibited significantly higher mean zones of inhibition of 18.33 mm, 27.70 mm, and 18.96 mm, respectively, against S. mutans at p<0.001 compared to L. acidophilus. In contrast, chlorhexidine showed 27.53 mm and 19.39 mm for S. mutans and L. acidophilus, respectively. Conclusion: Japanese green tea demonstrated superior antibacterial activity at its ethanolic concentration compared to other concentrations and the other two types of tea.","PeriodicalId":9054,"journal":{"name":"Biomedical and Pharmacology Journal","volume":"31 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Antimicrobial Efficiency of Different forms of Tea Extract (Camellia sinensis) against Cariogenic Pathogens\",\"authors\":\"V. Shivakumar, A. Tegginamani, N. Zain, Avita Rath, A. Zamzuri\",\"doi\":\"10.13005/bpj/2678\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Introduction: Dental caries is a frequent chronic infectious condition in the general population. Streptococcus mutans and Lactobacillus acidophilus play a significant part in the cause of dental caries. Various antimicrobials have been tried to prevent these microorganisms. Traditional herbal medicine extracted from plants has been employed as a remedy. Among them, tea leaf extract showed promising antimicrobial properties against infections. Aims: The current study was designed to assess the antibacterial activity of various types of tea extract against cariogenic microorganisms. Methods: S. mutans and L. acidophilus were grown in this in-vitro experimental study and maintained in their respective agars. Three different concentrations of 250 mg/ml aqueous, 250 mg/ml ethanolic, and 250 mg/ml aqueous with sugar solutions were prepared from Japanese green tea, Oolong Chinese tea, and Sabah black tea. Kirby-Bauer disc diffusion method was used to assess the antibacterial activity of tea extracts. As positive and negative control groups, 0.2% chlorhexidine and 1% DMSO were employed. The zone of inhibition was determined in millimetres following a 24-hour incubation period at 37 °C. Results: Japanese green tea at its three different concentrations exhibited significantly higher mean zones of inhibition of 18.33 mm, 27.70 mm, and 18.96 mm, respectively, against S. mutans at p<0.001 compared to L. acidophilus. In contrast, chlorhexidine showed 27.53 mm and 19.39 mm for S. mutans and L. acidophilus, respectively. Conclusion: Japanese green tea demonstrated superior antibacterial activity at its ethanolic concentration compared to other concentrations and the other two types of tea.\",\"PeriodicalId\":9054,\"journal\":{\"name\":\"Biomedical and Pharmacology Journal\",\"volume\":\"31 1\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2023-06-30\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biomedical and Pharmacology Journal\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.13005/bpj/2678\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"Pharmacology, Toxicology and Pharmaceutics\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biomedical and Pharmacology Journal","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.13005/bpj/2678","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"Pharmacology, Toxicology and Pharmaceutics","Score":null,"Total":0}
Antimicrobial Efficiency of Different forms of Tea Extract (Camellia sinensis) against Cariogenic Pathogens
Introduction: Dental caries is a frequent chronic infectious condition in the general population. Streptococcus mutans and Lactobacillus acidophilus play a significant part in the cause of dental caries. Various antimicrobials have been tried to prevent these microorganisms. Traditional herbal medicine extracted from plants has been employed as a remedy. Among them, tea leaf extract showed promising antimicrobial properties against infections. Aims: The current study was designed to assess the antibacterial activity of various types of tea extract against cariogenic microorganisms. Methods: S. mutans and L. acidophilus were grown in this in-vitro experimental study and maintained in their respective agars. Three different concentrations of 250 mg/ml aqueous, 250 mg/ml ethanolic, and 250 mg/ml aqueous with sugar solutions were prepared from Japanese green tea, Oolong Chinese tea, and Sabah black tea. Kirby-Bauer disc diffusion method was used to assess the antibacterial activity of tea extracts. As positive and negative control groups, 0.2% chlorhexidine and 1% DMSO were employed. The zone of inhibition was determined in millimetres following a 24-hour incubation period at 37 °C. Results: Japanese green tea at its three different concentrations exhibited significantly higher mean zones of inhibition of 18.33 mm, 27.70 mm, and 18.96 mm, respectively, against S. mutans at p<0.001 compared to L. acidophilus. In contrast, chlorhexidine showed 27.53 mm and 19.39 mm for S. mutans and L. acidophilus, respectively. Conclusion: Japanese green tea demonstrated superior antibacterial activity at its ethanolic concentration compared to other concentrations and the other two types of tea.
期刊介绍:
Biomedical and Pharmacology Journal (BPJ) is an International Peer Reviewed Research Journal in English language whose frequency is quarterly. The journal seeks to promote research, exchange of scientific information, consideration of regulatory mechanisms that affect drug development and utilization, and medical education. BPJ take much care in making your article published without much delay with your kind cooperation and support. Research papers, review articles, short communications, news are welcomed provided they demonstrate new findings of relevance to the field as a whole. All articles will be peer-reviewed and will find a place in Biomedical and Pharmacology Journal based on the merit and innovativeness of the research work. BPJ hopes that Researchers, Research scholars, Academician, Industrialists etc. would make use of this journal for the development of science and technology. Topics of interest include, but are not limited to: Biochemistry Genetics Microbiology and virology Molecular, cellular and cancer biology Neurosciences Pharmacology Drug Discovery Cardiovascular Pharmacology Neuropharmacology Molecular & Cellular Mechanisms Immunology & Inflammation Pharmacy.