水动力活化普通松木屑——培养菌株Gl4-16A灵芝的基质

IF 0.5 Q4 CHEMISTRY, MULTIDISCIPLINARY Journal of Siberian Federal University. Chemistry Pub Date : 2022-03-01 DOI:10.17516/1998-2836-0274
V. S. Fedorov
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引用次数: 0

摘要

本文报道了以普通松木木屑为基质培养灵芝Gl4-16A菌株的研究结果,木屑在空化水冲击分散剂中初步活化25分钟。研究发现,在灵芝Gl4-16A菌株的组成中引入(NH4)2SO4和Na2HPO4盐后,菌株在底物上的定殖效率更高。同时,担子菌在未添加盐的培养基上的平均生长速率为1.39±0.53 mm/d,在添加了氮盐的培养基上的平均生长速率为3.22±0.48 mm/d,并在第13天发生基质完全污染。结果表明,在含氮培养基上培养灵芝Gl4-16A菌株,其萃取物含量由4.37%增加到6.32%,其中水萃取物占65%以上。难水解多糖的含量减少了13.3%,而不可水解部分的含量增加了2.4%,即每有机质量的6.8%。易水解多糖(LGA)的含量下降了2.4%,但在有机质量方面变化不显著,生物转化前后LGA的比例均为15.7%左右。所获得的数据可以扩大木屑的使用领域,这是一个有前途的方向,无论是在化学工业还是制药工业
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Hydrodynamically Activated Sawdust of Common Pine Pinus sylvestris L.-substrate for Cultivation of the Strain Gl4–16A Ganoderma lucidum
The results of a study on the use of sawdust of common pine Pinus sylvestris L. with their preliminary activation in a cavitation water-impact dispersant for 25 minutes as a substrate for cultivating the Gl4–16A strain of Ganoderma lucidum are presented. It was found that the Gl4–16A strain of Ganoderma lucidum colonizes the substrate more efficiently with the introduction of (NH4)2SO4 and Na2HPO4 salts into its composition. At the same time, the average growth rate of basidiomycete on the substrate without the introduction of salts was 1.39±0.53 mm/day, and on the substrate with nitrogen salts – 3.22±0.48 mm/day, and complete fouling of the substrate occurred on day 13. It was found that during the cultivation of the Gl4–16A strain of Ganoderma lucidum on a substrate with nitrogen salts, the content of extractive substances in it increases from 4.37 % to 6.32 %, where more than 65 % is accounted for by substances extracted with water. There is a decrease in the content of difficult-to-hydrolyze polysaccharides by 13.3 %, and an increase in the non-hydrolyzable part by 2.4 %, which is 6.8 % per organic mass. The content of easily hydrolyzable polysaccharides (LGA) decreases by 2.4 %, but this change is not significant in terms of organic mass and the share of LGA both before and after bioconversion is about 15.7 %. The obtained data can expand the field of use of sawdust, which is a promising direction, both in the chemical and pharmaceutical industries
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