Cytogenetic Variability Analysis for Allium cepa L in Response to Green Synthesis Silver Nanoparticles

The present study was carried out on Allium cepa root tip to evaluate the cytoxicity of different concentrations of green synthesis silver nanoparticles (AgNPs). For this the root tips were treated with different concentrations of AgNPs (0.0, 5, 10, 20 and 40mg/L). The percentage of mitotic index was found to be significantly decreased as the concentration of the AgNPs increased except in 5mg/L concentration, where the mitotic index is higher thancontrol. The chromosomal abnormalities were also estimated. AgNPs induced a wide range of mitotic disturbances in the Allium root tips when compared to control. According to present findings 5mg/L of the AgNPs was found to enhance the rate of cell division when compare to control. On the contrary, higher of the AgNPs showed negative effects on mitotic division in root tip cells of onion. The SDS-PAGE analysis of the protein profile in root samples of AgNPs-treated plants revealed major changes than control. This experiment showed that the changes in protein profile may interrelated chromosomal abnormalities leading to mitotic arrest.