Isolation, Optimization and Application of Pigment Producing Bacteria from Soil

Color is the most crucial aspect of any item since it improves its attractiveness and acceptability. Different dyes are available in the market which makes their way to different industries. Synthetic colors are more reliable and less expensive than natural colors, but due to health hazards, few remain prohibited. In dye, food, pharmaceutical, cosmetic industries, widely use innocuous pigments from microbes. The work focuses on isolating the pigment-producing bacteria from soil and investigate several parameters that influence pigment synthesis. Bio colorant derived from A1 and A2 were applied on cotton fabric with pigment extracted by the solvent method. Different temperature, pH, incubation, and static and shaking conditions were investigated in this study. Biochemical and Molecular analysis identified both isolates A1 and A2 as Pseudomonas aeruginosa and SalinococcusI roseus. This work demonstrated pigment production was good under neutral pH and at shaking conditions. Both isolates showed different incubation times for pigment synthesis. Only Pseudomonas aeruginosa exhibited antibacterial action against the test pathogens E.coli and Staphylococcus aureus.