精选有益乳酸菌接种玉米和高粱青贮的效果

Vignolo Gm
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引用次数: 1

摘要

初步研究了发酵乳杆菌CRL2085单独或与同质发酵的嗜酸乳酸球球菌CRL2043联合使用对玉米和高粱青贮发酵及有氧稳定性的影响。为了将这些乳酸菌作为青贮的接种剂,对其进行了纤维分解、淀粉分解和抗真菌活性的筛选。准备实验室筒仓,以105 CFU/g接种,25℃发酵45天。发酵结束时,接种乳酸球球菌的玉米和高粱青贮pH最低,残余水溶性碳水化合物(WSC)较高。与发酵乳杆菌+酸活性Pediococcus接种的青贮相比,发酵乳杆菌+酸活性Pediococcus接种的青贮干物质(DM)损失和中性洗涤纤维(NDF)值更低,剩余WSC浓度更高,因此必须提高青贮消化率。单独接种发酵乳杆菌或联合接种酸乳酸球球菌可降低青贮酵母活性,提高好氧稳定性。PCR法测定乳酸菌作为接种剂在发酵过程中的存活情况。除了作为青贮接种剂具有良好的性能外,以前选择的对健康有益的乳酸菌可以通过发酵青贮作为载体在饲养场给牛
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Effect of Selected and Beneficial Lactic Acid Bacteria as Inoculants for Corn and Sorghum Silages
The preliminary effect of Lactobacillus fermentum CRL2085, alone or in combination with the homofermentative Pediococcus acidilactici CRL2043 on the fermentation and aerobic stability of corn and sorghum silages, was investigated. To be used as silages inoculants, these lactic acid bacteria (LAB) were screened for fibrolytic, amylolytic and antifungal activities. Laboratory silos were prepared, inoculated at 105 CFU/g and fermented during 45 days 25 ºC. At the end of fermentation, Pediococcus acidilactici-inoculated corn and sorghum silages exhibited the lowest pH and high residual water-soluble carbohydrates (WSC). Forage silages inoculated with Lactobacillus fermentum + Pediococcus acidilactici showed Lower Dry matter (DM) losses and Neutral Detergent Fiber (NDF) values, while higher residual WSC concentration than Lactobacillus fermentum-inoculated silages, thus silage digestibility must be improved. Yeast activity was impaired in silages inoculated with Lactobacillus fermentum alone or combined with Pediococcus acidilactici and aerobic stability improved. The survival of LAB used as inoculants during fermentation was suggested by PCR. Besides the good performance as silages inoculants, LAB previously selected as having health benefits may be delivered to cattle in feedlot through fermented silage as a vehicle
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