绿茶提取物对生西瓜金黄色葡萄球菌和肺炎克雷伯氏菌的治疗作用

Meat Research Pub Date : 2023-06-30 DOI:10.55002/mr.3.3.57
S. Biswas, M. Islam, T. Sarker, SS Islam, FT Meem, S Hassan :, S. Fariha
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摘要

本研究考察了不同浓度绿茶提取物对生绿茶中选择性微生物的影响。试验采用完全随机设计(CRD) 6个处理(T0 =0 ml绿茶;T1 =1毫升绿茶;T2 =2毫升绿茶;T3 =3毫升绿茶;T4 =4毫升绿茶,T5=5毫升绿茶,有三个重复。本实验采用滴平板法测定其抑菌活性。结果表明,当将绿茶与菌落形成单位从T0稳步减少到T5时,样品中的微生物数量下降。绿茶对大肠杆菌和表皮葡萄球菌的数量有显著影响(p<0.0001)。处理后的样品中大肠杆菌TVC的最小和最大种群在T5和T0分别为6×105 CFU/ml和45×105 CFU/ml,表皮葡萄球菌T5和T0分别为4×105 CFU/ml和10×105 CFU/ml。绿茶提取物对大肠杆菌和表皮葡萄球菌的抑菌活性差异极显著(p<0.0001)。根据这些结果,可以得出这样的结论:在生奶酪中添加5毫升的绿茶提取物会减少其微生物数量。对于金黄色葡萄球菌和肺炎克雷伯菌,用绿茶提取物处理的样品中微生物数量显著减少(p<0.0001)。
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Efficacy of green tea extract on Staphylococcus aureus and Klebsiella pneumonia of raw chevon
The efficacy of green tea extract with different strengths on selective microorganisms of raw chevon was investigated in this study. The experiment was conducted using completely randomized design (CRD) six treatments (T0 =0 ml of green tea; T1 =1 ml of green tea; T2 =2 ml of green tea; T3 =3 ml of green tea; T4 =4 ml of green tea and T5=5 ml of green tea) having three replications. Drop plate method was used for analyzing antimicrobial activity in this experiment. The outcome illustrated a declining microbial population in samples while incorporating green tea with colony-forming units steadily reducing from T0 to T5. There was a significant effect (p<0.0001) of green tea on the number of E. coli and Staphylococcus epidermidis. The minimum and maximum population of TVC of E. coli, in the treated sample were 6×105 CFU/ml in T5 and 45×105 CFU/ml in T0 respectively while for Staphylococcus epidermidis, it was 4×105 CFU/ml in T5 and 10×105 CFU/ml in T0 respectively. Green tea extracts showed equal growth suppression and extremely significant differences (p<0.0001) in their antimicrobial activity against both E. coli and Staphylococcus epidermidis. Given the results, it is possible to draw the conclusion that adding green tea extract at a level of 5 ml to raw chevon will diminish its microbial population. For Staphylococcus aureus and Klebsiella pneumonia were significant reduction (p<0.0001) of the microbes among samples treated with green tea extracts.
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