{"title":"枸杞子树皮乙醇提取物抗溃疡活性的体内和体外实验研究","authors":"M. Raju, R. Manisha, V. Reddy, M. Niharika","doi":"10.9734/ajrimps/2022/v11i130179","DOIUrl":null,"url":null,"abstract":"Aims: The present research is focused on screening in vivo anti-ulcer activity using pylorus ligation and ethanol induced ulcer model. \nStudy Design: Fourty eight rats, randomly divided into eight groups, were used in this study. Bark of Alstonia scholaris were air-dried, ground into fine powder and used in the preparation of an ethanolic extract. \nPlace and Duration of Study: Department of Pharmacology, Osmania University, Hyderabad between December 2020 and September 2021. \nMethodology: Ethanol related ulcer was induced using 1 mL/kg b.w, p.o. Treated rats received ethanolic extract of Alstonia scholaris at various doses of 200 and 400 mg/kg b.w. Ulcer index, % ulcer protection were calculated and histological studies were conducted at 6 hr after pylorous ligation respectively. \nResults: Pharmacological estimations were done by means of 200 mg/kg, b.w. and 400 mg/kg, b.w. The total acidity and free acidity were decreased, pH was increased and ulcer index was decreased by Ethanolic extract of Alstonia scholaris (EEAS 200 and 400 mg/kg b.w., p.o.) in pylorus ligation model. Treatment with EEAS (200 and 400 mg/kg b.w. p.o.) has significantly decreased the ulcer index by ethanol induced ulcer model. To appreciate the ligand-binding attraction of the dynamic ingredients of the excerpt, docking trainings were accomplished for natural compounds against protein data bank (PDB) ID: 5A5N, PDB ID: 6Q2T, PDB ID: 7MBX, PDB ID: 2FV5. The results revealed that vanillic acid, venoterpine, loganetin, dibutyl phthalate, guaia-3,9- diene, 3,6-Bis[2-methylphenyl]-2,5-dihydropyrrolo[3,4-c] pyrrole-1,4- dione, 2H-1-Benzopyran- 2-one,7-acetyl-8-[acetyloxy]-4, n-hexadecanoic acid, stigmasterol, diospyrolide, D- Friedoolean-14-en-3-one, betulin, lupeol acetate, pentanoic acid and standard drug omeprazole had shown highest glide scores with all the selected proteins which indicate a stronger receptor-ligand binding affinity. \nConclusion: From in vivo and in silico results it is evident that ethanolic bark extract of Alstonia scholaris possessed significant anti-ulcer activity.","PeriodicalId":8536,"journal":{"name":"Asian Journal of Research in Medical and Pharmaceutical Sciences","volume":"33 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2022-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":"{\"title\":\"Anti-ulcer Activity of Ethanolic Extract of Alstonia scholaris Bark: An in vivo and in silico Approach\",\"authors\":\"M. Raju, R. Manisha, V. Reddy, M. Niharika\",\"doi\":\"10.9734/ajrimps/2022/v11i130179\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Aims: The present research is focused on screening in vivo anti-ulcer activity using pylorus ligation and ethanol induced ulcer model. \\nStudy Design: Fourty eight rats, randomly divided into eight groups, were used in this study. Bark of Alstonia scholaris were air-dried, ground into fine powder and used in the preparation of an ethanolic extract. \\nPlace and Duration of Study: Department of Pharmacology, Osmania University, Hyderabad between December 2020 and September 2021. \\nMethodology: Ethanol related ulcer was induced using 1 mL/kg b.w, p.o. Treated rats received ethanolic extract of Alstonia scholaris at various doses of 200 and 400 mg/kg b.w. Ulcer index, % ulcer protection were calculated and histological studies were conducted at 6 hr after pylorous ligation respectively. \\nResults: Pharmacological estimations were done by means of 200 mg/kg, b.w. and 400 mg/kg, b.w. The total acidity and free acidity were decreased, pH was increased and ulcer index was decreased by Ethanolic extract of Alstonia scholaris (EEAS 200 and 400 mg/kg b.w., p.o.) in pylorus ligation model. Treatment with EEAS (200 and 400 mg/kg b.w. p.o.) has significantly decreased the ulcer index by ethanol induced ulcer model. To appreciate the ligand-binding attraction of the dynamic ingredients of the excerpt, docking trainings were accomplished for natural compounds against protein data bank (PDB) ID: 5A5N, PDB ID: 6Q2T, PDB ID: 7MBX, PDB ID: 2FV5. The results revealed that vanillic acid, venoterpine, loganetin, dibutyl phthalate, guaia-3,9- diene, 3,6-Bis[2-methylphenyl]-2,5-dihydropyrrolo[3,4-c] pyrrole-1,4- dione, 2H-1-Benzopyran- 2-one,7-acetyl-8-[acetyloxy]-4, n-hexadecanoic acid, stigmasterol, diospyrolide, D- Friedoolean-14-en-3-one, betulin, lupeol acetate, pentanoic acid and standard drug omeprazole had shown highest glide scores with all the selected proteins which indicate a stronger receptor-ligand binding affinity. \\nConclusion: From in vivo and in silico results it is evident that ethanolic bark extract of Alstonia scholaris possessed significant anti-ulcer activity.\",\"PeriodicalId\":8536,\"journal\":{\"name\":\"Asian Journal of Research in Medical and Pharmaceutical Sciences\",\"volume\":\"33 1\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2022-01-15\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Asian Journal of Research in Medical and Pharmaceutical Sciences\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.9734/ajrimps/2022/v11i130179\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Asian Journal of Research in Medical and Pharmaceutical Sciences","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.9734/ajrimps/2022/v11i130179","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Anti-ulcer Activity of Ethanolic Extract of Alstonia scholaris Bark: An in vivo and in silico Approach
Aims: The present research is focused on screening in vivo anti-ulcer activity using pylorus ligation and ethanol induced ulcer model.
Study Design: Fourty eight rats, randomly divided into eight groups, were used in this study. Bark of Alstonia scholaris were air-dried, ground into fine powder and used in the preparation of an ethanolic extract.
Place and Duration of Study: Department of Pharmacology, Osmania University, Hyderabad between December 2020 and September 2021.
Methodology: Ethanol related ulcer was induced using 1 mL/kg b.w, p.o. Treated rats received ethanolic extract of Alstonia scholaris at various doses of 200 and 400 mg/kg b.w. Ulcer index, % ulcer protection were calculated and histological studies were conducted at 6 hr after pylorous ligation respectively.
Results: Pharmacological estimations were done by means of 200 mg/kg, b.w. and 400 mg/kg, b.w. The total acidity and free acidity were decreased, pH was increased and ulcer index was decreased by Ethanolic extract of Alstonia scholaris (EEAS 200 and 400 mg/kg b.w., p.o.) in pylorus ligation model. Treatment with EEAS (200 and 400 mg/kg b.w. p.o.) has significantly decreased the ulcer index by ethanol induced ulcer model. To appreciate the ligand-binding attraction of the dynamic ingredients of the excerpt, docking trainings were accomplished for natural compounds against protein data bank (PDB) ID: 5A5N, PDB ID: 6Q2T, PDB ID: 7MBX, PDB ID: 2FV5. The results revealed that vanillic acid, venoterpine, loganetin, dibutyl phthalate, guaia-3,9- diene, 3,6-Bis[2-methylphenyl]-2,5-dihydropyrrolo[3,4-c] pyrrole-1,4- dione, 2H-1-Benzopyran- 2-one,7-acetyl-8-[acetyloxy]-4, n-hexadecanoic acid, stigmasterol, diospyrolide, D- Friedoolean-14-en-3-one, betulin, lupeol acetate, pentanoic acid and standard drug omeprazole had shown highest glide scores with all the selected proteins which indicate a stronger receptor-ligand binding affinity.
Conclusion: From in vivo and in silico results it is evident that ethanolic bark extract of Alstonia scholaris possessed significant anti-ulcer activity.