{"title":"采用粒径均匀的胶体几丁质粉作为底物，改进斜纹夜蛾几丁质酶抑制剂的生物测定方法","authors":"T. Nitoda, H. Kurumatani, H. Kanzaki, K. Kawazu","doi":"10.1002/(SICI)1096-9063(199905)55:5<563::AID-PS972>3.0.CO;2-#","DOIUrl":null,"url":null,"abstract":"A previously reported bioassay method for Spodoptera litura chitinase inhibitors has been improved by use of colloidal chitin powder with a uniform particle size. This improvement made the assay four times more sensitive. Detection of three active supernatants by screening of supernatants and cell extracts from 135 fermentation broths has proved the efficiency of this improved method.","PeriodicalId":19985,"journal":{"name":"Pesticide Science","volume":"58 1","pages":"563-565"},"PeriodicalIF":0.0000,"publicationDate":"1999-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"10","resultStr":"{\"title\":\"Improved bioassay method for Spodoptera litura chitinase inhibitors using a colloidal chitin powder with a uniform particle size as substrate\",\"authors\":\"T. Nitoda, H. Kurumatani, H. Kanzaki, K. Kawazu\",\"doi\":\"10.1002/(SICI)1096-9063(199905)55:5<563::AID-PS972>3.0.CO;2-#\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"A previously reported bioassay method for Spodoptera litura chitinase inhibitors has been improved by use of colloidal chitin powder with a uniform particle size. This improvement made the assay four times more sensitive. Detection of three active supernatants by screening of supernatants and cell extracts from 135 fermentation broths has proved the efficiency of this improved method.\",\"PeriodicalId\":19985,\"journal\":{\"name\":\"Pesticide Science\",\"volume\":\"58 1\",\"pages\":\"563-565\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1999-05-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"10\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Pesticide Science\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1002/(SICI)1096-9063(199905)55:5<563::AID-PS972>3.0.CO;2-#\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Pesticide Science","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1002/(SICI)1096-9063(199905)55:5<563::AID-PS972>3.0.CO;2-#","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Improved bioassay method for Spodoptera litura chitinase inhibitors using a colloidal chitin powder with a uniform particle size as substrate
A previously reported bioassay method for Spodoptera litura chitinase inhibitors has been improved by use of colloidal chitin powder with a uniform particle size. This improvement made the assay four times more sensitive. Detection of three active supernatants by screening of supernatants and cell extracts from 135 fermentation broths has proved the efficiency of this improved method.
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