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Effect of paraoxon‐methyl and parathion‐methyl on DNA in human lymphocytes and protective action of vitamin C 甲基对硫磷和甲基对硫磷对人淋巴细胞DNA的影响及维生素C的保护作用
Pub Date : 1999-12-01 DOI: 10.1002/(SICI)1096-9063(199912)55:12<1182::AID-PS75>3.0.CO;2-#
J. Błasiak, J. Kowalik
The molecular basis of the genotoxicity of the commonly used organophosphorus insecticide, parathion-methyl is poorly understood and there is a lack of information on the possible effects of its metabolic conversion products. In the present work the action of parathion-methyl and its immediate metabolite paraoxon-methyl on DNA in human lymphocytes was compared using the comet assay. Parathion-methyl at 25 and 75 µM did not cause any significant changes but at 200 µM a significant increase in the tail moment was observed as compared with the control. Paraoxon-methyl at 25, 75 and 200 µM evoked dose-dependent DNA damage measured as a significant increase in comet tail moment of lymphocytes. The change evoked by paraoxon-methyl at 200 µM was much more pronounced than that by parathion-methyl at the same concentration. To search for the mechanism underlying the observed effect, the action of a well-known antioxidant, vitamin C, along with parathion-methyl and paraoxon-methyl was studied. The vitamin at 10 and 50 µM reduced the DNA-damaging activity of paraoxon-methyl at all its concentrations. The results indicate that the reported genotoxic effects of parathion-methyl could be mainly attributed to its metabolite paraoxon-methyl. The protective action of vitamin C suggests that paraoxon-methyl may cause oxidative DNA damage. © 1999 Society of Chemical Industry
人们对常用的有机磷杀虫剂甲基对硫磷遗传毒性的分子基础了解甚少,对其代谢转化产物可能产生的影响也缺乏信息。本文用彗星法比较了甲基对硫磷及其直接代谢物甲基对硫磷对人淋巴细胞DNA的作用。甲基对硫磷在25和75µM时没有引起任何显著变化,但在200µM时,与对照相比,尾部力矩显著增加。25、75和200µM的甲基对氧磷引起了剂量依赖性的DNA损伤,淋巴细胞彗尾时刻显著增加。在200µM浓度下,甲基对硫磷引起的变化比相同浓度的甲基对硫磷引起的变化更明显。为了寻找观察到的效应背后的机制,研究了一种众所周知的抗氧化剂维生素C与甲基对硫磷和甲基对氧磷的作用。10µM和50µM的维生素降低了所有浓度的甲基对氧磷的dna损伤活性。结果表明,甲基对硫磷的遗传毒性作用可能主要归因于其代谢物甲基对硫磷。维生素C的保护作用提示甲基对氧磷可能引起DNA氧化损伤。©1999化学工业学会
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引用次数: 16
In-vitro study on the effect of pesticides on neuronal activity 农药对神经元活性影响的体外研究
Pub Date : 1999-10-01 DOI: 10.1002/(SICI)1096-9063(199910)55:10<1037::AID-PS45>3.0.CO;2-#
J. Doczi, I. Világi, I. Banczerowski-Pelyhe, F. Örsi, Z. Szentpétery
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引用次数: 5
Communication to the editor piperonyl butoxide-mediated inhibition of cytochrome P450-catalysed insecticide metabolism: A rational approach 胡椒酰丁醇介导的抑制细胞色素p450催化的杀虫剂代谢:一种合理的方法
Pub Date : 1999-10-01 DOI: 10.1002/PS.2780551008
G. Keserü, I. Kolossváry, B. Bertók
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引用次数: 0
Review Methods of analysis of dithiocarbamate pesticides: A review 二硫代氨基甲酸酯类农药分析方法综述
Pub Date : 1999-10-01 DOI: 10.1002/PS.2780551002
A. Malik, W. Faubel
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引用次数: 5
Monitoring of the photochemical degradation of metamitron and imidacloprid by micellar electrokinetic chromatography and differential-pulse polarography 胶束电动色谱和差脉冲极谱法监测亚胺和吡虫啉的光化学降解
Pub Date : 1999-09-01 DOI: 10.1002/(SICI)1096-9063(199909)55:9<949::AID-PS48>3.0.CO;2-#
J. Cacho, I. Fierro, L. Debán, M. Vega, R. Pardo
The photochemical degradation of metamitron (4-amino-4,5-dihydro-3-methyl-6-phenyl-1,2,4-triazin-5-one) and imidacloprid (1-(6-chloro-3-pyridylmethyl)-N-nitroimidazolidin-2-ylideneamine) has been investigated by differential-pulse polarography (DPP) and micellar electrokinetic chromatography (MEKC); the degradation pathways of these pesticides were elucidated and their degradation products proposed.  The electrochemical study of imidacloprid by DPP at different pH values demonstrated the occurrence of two different reduction processes; at pH 6.8, two peaks at −0.90 V and −1.38 V, respectively, were obtained, which are related to the photochemical reduction processes. The photochemical degradation of imidacloprid caused by sunlight was polarographically monitored and its degradation products elucidated. The polarographic reduction of deaminometamitron (obtained by photochemical reduction of metamitron) yielded two peaks at −0.62 and −1.37 V, which are related to the reduction of the CN bonds. The effect of sunlight on the reduction of metamitron was monitored by DPP, and an increase of the concentration of the degradation products was observed with time.  MEKC with UV-visible detection was used to separate the pesticides and the products of their photochemical degradation, which were identified in combination with DPP. © 1999 Society of Chemical Industry
采用差分脉冲极谱法(DPP)和胶束电动色谱法(MEKC)研究了异氨氮(4-氨基-4,5-二氢-3-甲基-6-苯基-1,2,4-三嗪-5- 1)和咪氯啉(1-(6-氯-3-吡啶基甲基)- n-硝基咪唑烷-2-酰基胺)的光化学降解;阐明了这些农药的降解途径,提出了它们的降解产物。不同pH值下DPP对吡虫啉的电化学研究表明,发生了两种不同的还原过程;在pH 6.8时,分别在−0.90 V和−1.38 V处出现两个峰,这两个峰与光化学还原过程有关。采用极谱法对吡虫啉的光化学降解过程进行了监测,并对其降解产物进行了分析。通过光化学还原得到脱胺异氨氮的极谱还原,在−0.62和−1.37 V处产生两个峰,这与CN键的还原有关。利用DPP监测光照对变氮还原的影响,观察到降解产物浓度随时间的增加而增加。采用MEKC紫外可见检测分离农药及其光化学降解产物,并结合DPP进行鉴定。©1999化学工业学会
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引用次数: 10
Field-simulator study of insecticide resistance conferred by esterase-, MACE- and kdr-based mechanisms in the peach-potato aphid, Myzus persicae (Sulzer) 桃薯蚜酯酶、MACE和kdr抗性田间模拟研究
Pub Date : 1999-08-01 DOI: 10.1002/(SICI)1096-9063(199908)55:8<810::AID-PS19>3.0.CO;2-#
S. Foster, A. Devonshire
Insecticide sprays were applied to Myzus persicae (Sulzer) populations carrying various combinations of three insecticide resistance mechanisms (esterase-based metabolic resistance and two target site mechanisms, known as MACE and kdr), supported on host plants growing in field simulator cages. The study showed that MACE confers extreme resistance to pirimicarb and triazamate (carbamate insecticides) but not to deltamethrin + heptenophos (16 + 1) (Decisquick) or dimethoate (an organophosphorus insecticide). Resistance to dimethoate depends solely on levels of esterase-based resistance, while resistance to Decisquick depends on kdr and esterase. None of the four insecticides is effective against aphids carrying MACE combined with extreme esterase-based resistance. This knowledge, in association with current monitoring of the mechanisms, will play an important role in making decisions on insecticide use against M persicae in the UK. © 1999 Society of Chemical Industry
以田间模拟网箱中生长的寄主植物为支撑,对携带3种不同抗性机制(酯酶代谢抗性和MACE和kdr两种靶点抗性机制)的桃蚜(Myzus persicae, Sulzer)种群进行了杀虫剂喷洒。研究表明,MACE对吡乐威和三嗪酯(氨基甲酸酯类杀虫剂)具有极强的抗性,但对溴氰菊酯+七磷(16 + 1)(Decisquick)或乐果(一种有机磷杀虫剂)则没有。对乐果的耐药仅取决于酯酶基础的耐药水平,而对Decisquick的耐药取决于kdr和酯酶。这四种杀虫剂对携带MACE的蚜虫没有一种有效,而且这些蚜虫具有极强的酯酶抗性。这一知识与目前对机制的监测相结合,将在英国制定针对桃蚜的杀虫剂使用决策中发挥重要作用。©1999化学工业学会
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引用次数: 26
Improved bioassay method for Spodoptera litura chitinase inhibitors using a colloidal chitin powder with a uniform particle size as substrate 采用粒径均匀的胶体几丁质粉作为底物,改进斜纹夜蛾几丁质酶抑制剂的生物测定方法
Pub Date : 1999-05-01 DOI: 10.1002/(SICI)1096-9063(199905)55:5<563::AID-PS972>3.0.CO;2-#
T. Nitoda, H. Kurumatani, H. Kanzaki, K. Kawazu
A previously reported bioassay method for Spodoptera litura chitinase inhibitors has been improved by use of colloidal chitin powder with a uniform particle size. This improvement made the assay four times more sensitive. Detection of three active supernatants by screening of supernatants and cell extracts from 135 fermentation broths has proved the efficiency of this improved method.
先前报道的斜纹夜蛾几丁质酶抑制剂的生物测定方法已通过使用具有均匀粒径的胶体几丁质粉得到改进。这种改进使化验的灵敏度提高了四倍。通过对135个发酵液上清液和细胞抽提液的筛选,检测出3种活性上清液,验证了改进方法的有效性。
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引用次数: 10
Differentiating between physical and chemical constraints on pesticide and water movement into and out of soil aggregates 区分农药和水分进出土壤团聚体的物理和化学限制
Pub Date : 1999-05-01 DOI: 10.1002/(SICI)1096-9063(199905)55:5<524::AID-PS937>3.0.CO;2-#
A. Johnson, Robert J Bettinson, Richard J. Williams
A laboratory experiment comparing the movement of 3 H 2 O and [ 14 C]isoproturon into and release from soil aggregates is described. Small aggregates (2.0-2.4mm) were prepared from a clay topsoil and maintained at three different initial moisture conditions. A small volume of the radioisotope solution was introduced prior to bathing the aggregates in a 2mM CaCl 2 solution to represent new rainwater. Whilst the 3 H 2 O was imbibed by the air-dry aggregates, the pesticide did not follow the water but remained on the surface of the aggregates. This may be related to its sorptive properties and an excess of sorption sites on the sorbent with respect to the sorbate. Increasing the length of exposure of the moist aggregates to [ 14 C]isoproturon reduced the initial release of the compound into the bathing solution, probably due to diffusion (retarded by sorption) into the aggregates. The diffusion model described by Crank and a non-equilibrium desorption model were used to analyse the 3 H 2 O and [ 14 C]isoproturon release curves. This showed that the release of 3 H 2 O from the dry aggregates was controlled by diffusion. The release of isoproturon was probably controlled by non-equilibrium sorption/desorption from air-dry aggregates and by a combination of non-equilibrium sorption/desorption and diffusion from wet aggregates.
本文描述了一项比较3h2o和[14c]异proturon进入土壤团聚体和从土壤团聚体释放的室内实验。从粘土表层土中制备了小团聚体(2.0-2.4mm),并在三种不同的初始水分条件下保持。在将聚集体浸泡在2mM氯化钙溶液中之前,引入少量放射性同位素溶液,以代表新的雨水。当3h2o被空气干燥的聚集体吸收时,农药没有跟随水而停留在聚集体的表面。这可能与它的吸附特性和吸附剂上相对于山梨酸盐的过量吸附位点有关。增加潮湿的聚集体暴露于[14 C]异proturon的时间,减少了化合物在沐浴液中的初始释放,可能是由于扩散(被吸附延迟)到聚集体中。采用曲克扩散模型和非平衡解吸模型分析了3h2o和[14c]异丙隆的释放曲线。说明干团聚体中3h2o的释放受扩散控制。异proturon的释放可能受风干团聚体的非平衡吸附/解吸和湿团聚体的非平衡吸附/解吸和扩散的共同控制。
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引用次数: 19
Exposure assessment of professional pesticide users during treatment of potato fields 马铃薯田处理期间专业农药使用者的暴露评估
Pub Date : 1999-04-01 DOI: 10.1002/(SICI)1096-9063(199904)55:4<467::AID-PS924>3.0.CO;2-#
F. Vercruysse, S. Drieghe, W. Steurbaut, W. Dejonckheere
In this study four different mixing/loading and application practices in potato fields were monitored for exposure of operators to pesticides. Each operation - mixing, loading, and application - was measured individually in order to assess its relative contribution to the total exposure value. Inhalation exposure was measured by trapping the pesticides with a sorbent tube while sampling the air around the operator's face. Dermal deposition, which was measured by means of cotton gloves on the hands and by attaching patches to the operator's clothing, was the main contributor to the total exposure. Dermal deposition on the hands during mixing and loading exceeded all other dermal values. The experimental results are compared with the results obtained by the exposure assessment model PHED V1.1. This model gives an underestimation of the levels of operator exposure during mixing, loading and application.
本研究监测了马铃薯田4种不同的混合/装载和施用方式对操作人员农药暴露的影响。每个操作-混合,加载和应用-被单独测量,以评估其对总暴露值的相对贡献。吸入暴露的测量方法是用吸附剂管捕获农药,同时对操作人员面部周围的空气进行采样。皮肤沉积是总暴露的主要因素,皮肤沉积是通过在手上戴上棉质手套和在操作者的衣服上贴贴片来测量的。在混合和装载过程中,手上的皮肤沉积超过了所有其他皮肤值。实验结果与PHED V1.1暴露评估模型的结果进行了比较。该模型低估了混合、装载和应用过程中操作人员的暴露水平。
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引用次数: 23
Vertebrate wildlife incidents with pesticides: a european survey 与杀虫剂有关的脊椎动物野生动物事件:一项欧洲调查
Pub Date : 1999-01-01 DOI: 10.1002/(SICI)1096-9063(199901)55:1<47::AID-PS859>3.0.CO;2-#
G. D. Snoo, Nathalie M I Scheidegger, F. Jong
A survey was carried out to investigate terrestrial wildlife incidents with pesticides in 18 European countries over the period 1990-1994. Only in seven countries does a systematic incident registration system exist. Compared with the other countries, relatively high numbers of incidents were registered in France, the Netherlands and the United Kingdom. Over 1000 incidents were investigated to establish their causes: approved use, misuse or deliberate abuse, and the compounds, species and mode of application involved. It was found that most registered incidents are due to deliberate abuse. Approved use is responsible for only a minor fraction of the incidents, and these are due to particular practices such as use of treated seed, bait or wood preservatives and the spraying of grassland. Hardly any incidents were due to crop-spraying. The reason why so few incidents are registered for normal crop-spraying is discussed: do they not occur, or are the casualties not registered? It is doubtful whether incident registration is a reliable instrument for obtaining a proper understanding of the occurrence of the side-effects of agricultural pesticide use.
在1990-1994年期间,对18个欧洲国家的陆生野生动物杀虫剂事件进行了调查。只有7个国家有系统的事故登记制度。与其他国家相比,法国、荷兰和英国登记的事件数量相对较高。调查了1000多起事件,以确定其原因:批准使用、误用或故意滥用,以及所涉及的化合物、种类和应用方式。调查发现,大多数已登记的事件都是蓄意虐待造成的。经批准的使用仅占事故的一小部分,这些事故是由于使用处理过的种子、诱饵或木材防腐剂以及草地喷洒等特殊做法造成的。几乎没有任何事故是由于喷洒农作物造成的。讨论了为什么很少有事件被登记为正常的作物喷洒:它们没有发生,或者伤亡没有登记?值得怀疑的是,事件登记是否是一种可靠的工具,可以正确地了解农药使用副作用的发生情况。
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引用次数: 56
期刊
Pesticide Science
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