Simultaneous multiwavelength detection based on surface plasmon resonance technique

The present article covers a novel surface plasmon resonance (SPR) instrument based on simultaneous multiwavelength detection. The sensing principle of a traditional SPR device is based on fixing a discrete excitation wavelength and modulating the angle of incident light. The SPR-reflected spectra are shown in terms of reflected light intensity versus angle of incident light. The instrument presented here is designed on the basis of fixing the angle of incident light and measuring the change of resonant wavelength in the wavelength range of 400–800 nm. The SPR spectra are shown in terms of reflected light intensity versus wavelength of the incident light. The intensity of the reflected light is the minimum at the resonant wavelength. With the aid of the device, some chemical sensors and immunosensors, such as the thiolate chemical sensor, the fibrin immunosensor, and the albumin immunosensor were developed. The kinetic processes of formation of various sensing monolayers were studied in real time through observing the change of resonant wavelength. © 2000 John Wiley & Sons, Inc. Lab Robotics and Automation 12:104–107, 2000