{"title":"梭子鱼55个核糖体DNA的分子细胞遗传学特征","authors":"Mai Watanabe, N. Tsurusaki, Souichirou Kubota","doi":"10.11352/SCR.12.51","DOIUrl":null,"url":null,"abstract":"Entire 5S ribosomal DNA (rDNA), including 120 bp of rRNA gene and 142 bp of non-transcribed spacer (NTS) regions of Psathyropus tenuipes (Arachnida, Opiliones, Sclerosomatidae), a harvestman which usually bears several B-chromosomes, was sequenced and mapped on the spermatogonial metaphases using fluorescence insitu hybridization (FISH). The signals of 5S rDNA were detected on the terminal region of one pair of C-bandnegative autosomal chromosomes, showing that the 5S rDNA cluster is localized to A chromosome in this species, but not to B chromosome known as a rDNA carrier in other arthropods.","PeriodicalId":10221,"journal":{"name":"Chromosome science","volume":"53 1","pages":"51-53"},"PeriodicalIF":0.0000,"publicationDate":"2009-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"2","resultStr":"{\"title\":\"Molecular cytogenetic characterization of 55 ribosomal DNA in the harvestman Psathyropus tenuipes (Arachnida : Opiliones)\",\"authors\":\"Mai Watanabe, N. Tsurusaki, Souichirou Kubota\",\"doi\":\"10.11352/SCR.12.51\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Entire 5S ribosomal DNA (rDNA), including 120 bp of rRNA gene and 142 bp of non-transcribed spacer (NTS) regions of Psathyropus tenuipes (Arachnida, Opiliones, Sclerosomatidae), a harvestman which usually bears several B-chromosomes, was sequenced and mapped on the spermatogonial metaphases using fluorescence insitu hybridization (FISH). The signals of 5S rDNA were detected on the terminal region of one pair of C-bandnegative autosomal chromosomes, showing that the 5S rDNA cluster is localized to A chromosome in this species, but not to B chromosome known as a rDNA carrier in other arthropods.\",\"PeriodicalId\":10221,\"journal\":{\"name\":\"Chromosome science\",\"volume\":\"53 1\",\"pages\":\"51-53\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2009-07-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"2\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Chromosome science\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.11352/SCR.12.51\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Chromosome science","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.11352/SCR.12.51","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Molecular cytogenetic characterization of 55 ribosomal DNA in the harvestman Psathyropus tenuipes (Arachnida : Opiliones)
Entire 5S ribosomal DNA (rDNA), including 120 bp of rRNA gene and 142 bp of non-transcribed spacer (NTS) regions of Psathyropus tenuipes (Arachnida, Opiliones, Sclerosomatidae), a harvestman which usually bears several B-chromosomes, was sequenced and mapped on the spermatogonial metaphases using fluorescence insitu hybridization (FISH). The signals of 5S rDNA were detected on the terminal region of one pair of C-bandnegative autosomal chromosomes, showing that the 5S rDNA cluster is localized to A chromosome in this species, but not to B chromosome known as a rDNA carrier in other arthropods.
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