巴基斯坦旁遮普省北部耐多药结核病患者鲍曼不动杆菌分离株的分子特征

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摘要

呼吸道感染(RTIs)是发展中国家最常见和最严重的传染病。鲍曼不动杆菌是人类呼吸道感染的致病菌。报告指出,鲍曼不动杆菌与结核分枝杆菌的联合感染增加了耐多药结核病(MDR-TB)阳性患者的健康并发症,并对患病人群造成致命损害。本研究对来自北旁遮普省的106例耐多药结核病阳性患者的痰样本进行了研究。采用选择性培养基从耐多药结核病患者痰液中分离鲍曼不动杆菌,通过氧化酶和过氧化氢酶鉴定进行初步筛选,然后进行显微镜检查。随后,再次选择10株鲍曼不动杆菌疑似分离株,采用圆盘扩散法进一步鉴定耐多药耐药性。准确测定抗生素对抗菌药物的抗菌谱。其中8株对左氧氟沙星耐药,采用bla-OXA-51引物进行分子鉴定。106株中仅有3株(2.83%)被证实为鲍曼不动杆菌耐多药菌株。这些结果表明耐多药鲍氏杆菌与巴基斯坦北部旁遮普省耐多药结核病患者共存。在旁遮普北部地区,在住院患者中发现了与鲍曼不动杆菌合并感染的耐多药结核病的比例较高(3例)。这些发现可能表明不卫生的医院环境或做法导致合并感染。
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Molecular characterization of Acinetobacter baumannii isolated from MDR-TB patients of Northern Punjab, Pakistan
Respiratory tract infections (RTIs) are the most common and severe infectious diseases in developing countries. Acinetobacter baumannii is the bacterium known as causative organism for respiratory tract infections in human populations. The Report suggests that co-infection of Acinetobacter baumannii with Mycobacterium tuberculosis together increases health complications in multidrug resistant tuberculosis (MDR-TB) positive patients and creates fatal damage to the ailing population. In our study, 106 sputum samples of MDR-TB positive patients from Northern Punjab were studied. The isolation of A. baumannii from sputum of MDR-TB patients was done on selective media and initially screened by Oxidase and Catalase based identification followed by microscopic examination. Afterward, only ten suspected isolates of A. baumannii were again selected for further characterization for MDR by using Disc diffusion method. Antibiograms against number of antibiotics were accurately determined. Of these 10 isolates, 8 sample were found resistant to levofloxacin and subjected to molecular characterization using bla-OXA-51 primers. Only 3 out of 106 (2.83 %) isolates were confirmed as MDR strains of A. baumannii. These results show the coexistence of MDR A. bauminnii with MDR-TB patients of Northern Punjab, Pakistan. In Northern Punjab regions a higher percentage (3 cases) of MDR- TB were detected, which were co-infected with Acinetobacter baumannii among hospitalized patients. These findings may show unhygienic hospital environment or practices which leads to the co-infection.
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