The Effect of Sports Activity and Vitamin D Intake on Serum Eutoxin-1 and Superoxide Dismutase Levels in Overweight Women with Vitamin D Deficiency: A Clinical Trial Study

Introduction: Being overweight and having vitamin D deficiency are associated with oxidative stress and low-grade chronic inflammation, resulting in various diseases. In the present study, changes in the levels of Eutoxin-1 and superoxide dismutase (SOD) were evaluated after 8 weeks of high-intensity interval resistance training (HIIRT) and vitamin D intake in overweight women with vitamin D deficiency. Method: In this clinical trial study, 52 overweight women with vitamin D deficiency were purposefully selected and then randomly assigned to exercise, vitamin D, exercise+vitamin D (combined), and control groups. HIIRT was performed 3 times a week in three sets with an intensity of 80% of a maximum repetition. Vitamin D was taken at a dose of 50,000 units weekly. Data were analyzed using paired t-test, one-way ANOVA, and Kruskal-Wallis tests with a significant level of less than 0.05. Results: After 8 weeks, SOD activity increased in the experimental groups, and the level of Eutoxin-1 decreased significantly. In addition, the rate of changes in SOD and Eutoxin-1 levels in the combined group was higher than those in the vitamin D group; however, no significant difference was observed between the exercise and combination groups (P<0.05). Conclusion: It seems that HIIRT and vitamin D intake can increase SOD activity and up-regulate Eutoxin-1 in overweight women. Moreover, co-administration of HIIRT with vitamin D intake can have stronger effects in reducing the inflammatory-oxidative effects of overweight and vitamin D deficiency by increasing SOD and decreasing Eutoxin-1, compared to the intervention of vitamin D consumption.