羊奶中大肠杆菌-葡萄糖醛酸酶阳性的发生率

Zorica Voșgan, A. Domuța, Stela Jelea, Lucia Mihălescu, F. Pop
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摘要

自1976年以来,关于β -葡萄糖醛酸酶在环境、食物、水等来源中鉴定大肠杆菌的敏感性和特异性的论文陆续发表。在这项研究中,我们对羊奶中大肠杆菌I²-葡萄糖醛酸酶阳性的发生率进行了回顾,羊奶是在哺乳期间通过手工挤奶获得的:春、夏、秋。牛奶中大肠杆菌的存在被认为既是一种健康指标,也是一种能够引起食物中毒的致病因素。采用TBX培养基,在44℃下培养典型蓝色菌落,检测大肠杆菌I²-葡萄糖酸苷酶阳性。分析发现,在每天挤奶三次的春季,产奶中没有大肠杆菌;在产奶量较低且每天挤奶一次的秋季,也观察到同样的情况。在较高温度条件下,泌乳中期(7 ~ 8月)羊奶中大肠杆菌I²-葡萄糖醛酸酶阳性的平均载量为66.67 CFU/ml。在此期间,在山区进行挤奶,那里的动物在夏季进行放牧。显微镜检查也证实了大肠杆菌的存在。在炎热的天气里,应注意卫生,牛奶应立即冷却,因为大肠杆菌可能是食物中毒的来源。一个
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Incidence of Escherichia coli - Glucuronidase Positive on Goat Milk
Papers on beta- glucuronidase sensitivity and specificity for identifying Escherichia coli in sources of environment, food, water, etc. have been published since 1976. In this study we conducted a review of the incidence of E. coli I² - glucuronidase -positive in goat milk, obtained by hand milking throughout the lactation: spring, summer, autumn. The presence of E. coli in milk is considered both as a health indicator and a pathogenic factor capable of causing food poisoning. The determination of the E. coli I²-glucuronidase-positive was carried using TBX medium by cultivating colonies typical blue at 44 0 C. The absence of E. coli in milk yielded during the spring, when the animal milking is done three times a day, was found in the performed analyses; the same was observed during fall, when the milk production is lower and the milking is done once a day. The load of E. coli I²-glucuronidase-positive was averaging 66.67 CFU/ml of goat milk, during the middle lactation period (July-August), in conditions of higher temperature. During this period, milking is done in the mountain zone, where the transhumance of animals takes place in summer. The presence of the species E. coli was also confirmed by microscopic examination. Attention should be paid to hygiene and milk should be immediately cooled, during hot weather, as E. coli can be a source of food poisoning. Â
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