Kanokwan Jitpimai, L. Ngiwsara, Weeranuch Lang, Thanawan Panichpat, Ratchanee Mingma, Jisnuson Svasti, J. Wongchawalit
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The quantitative analysis revealed that the highest total phenolic compounds were obtained in MVBS70 with 1,186.37 mg/g gallic acid equivalent. Levodopa (L-Dopa), a major phytochemical in Mucuna seed, was found in EVBS70, MVBS70, MVBS95 and HVBS but not in EVBS95. Since the extraction by EVBS95 appeared an oil-liked substance (EVBS95oil), four fatty acids including palmitic acid, linoleic acid, oleic acid and stearic acid were detected by Gas Chromatography-Mass Spectrometry. The fractions of EVBS70, EVBS95, and MVBS95 exhibited an antibacterial activity for Bacillus cereus, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae and Salmonella typhi. Furthermore, the radical scavenging assay using 1,1-diphenyl-2-picrylhydrazyl as a free radical demonstrated that all extracts presented a higher activity against DPPH than that of the commercially available as L-Dopa, gallic acid, quercetin and catechin. Additionally, all extracts exhibited a dosedependent cytotoxic effect towards A549, HepG2 and SW480 cell lines, while EVBS70 showed the most potent cytotoxic effect. All extracts except HVBS showed the greatest effect on SW480 with IC50 values ranging from 105-205 μg/ml. This study provides additional effects of the alcoholic extracts on various pharmaceutical activities, in which velvet bean in Thailand is a promising economic plant for medical applications and industrial purposes.","PeriodicalId":9884,"journal":{"name":"Chiang Mai Journal of Science","volume":null,"pages":null},"PeriodicalIF":0.6000,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Evaluation of Alcoholic Extracts of Mucuna pruriens (L.) DC. Var. utilis for Antibacterial, Antioxidant and Cytotoxic Activities toward Human Cancer Cell Lines\",\"authors\":\"Kanokwan Jitpimai, L. 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The quantitative analysis revealed that the highest total phenolic compounds were obtained in MVBS70 with 1,186.37 mg/g gallic acid equivalent. Levodopa (L-Dopa), a major phytochemical in Mucuna seed, was found in EVBS70, MVBS70, MVBS95 and HVBS but not in EVBS95. Since the extraction by EVBS95 appeared an oil-liked substance (EVBS95oil), four fatty acids including palmitic acid, linoleic acid, oleic acid and stearic acid were detected by Gas Chromatography-Mass Spectrometry. The fractions of EVBS70, EVBS95, and MVBS95 exhibited an antibacterial activity for Bacillus cereus, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae and Salmonella typhi. Furthermore, the radical scavenging assay using 1,1-diphenyl-2-picrylhydrazyl as a free radical demonstrated that all extracts presented a higher activity against DPPH than that of the commercially available as L-Dopa, gallic acid, quercetin and catechin. 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引用次数: 0
摘要
Mucuna pruriens L. DC。鹿茸豆,一种药用印度植物,长期用于治疗包括帕金森病在内的一些神经系统疾病。这种植物富含植物化学化合物,被认为可以提供更多的药物治疗,但这些细节,特别是泰国品种的丝绒豆种子,目前还不清楚。本研究对丝绒豆种子提取物对三种人类癌细胞的抑菌、抗氧化和细胞毒活性进行了研究。采用70%乙醇(EVBS70)和95%乙醇(EVBS95)、70%甲醇(MVBS70)和95%甲醇(MVBS95)和盐酸(HVBS)进行提取。定量分析表明,MVBS70中总酚类化合物含量最高,其没食子酸当量为1186.37 mg/g。在EVBS70、MVBS70、MVBS95和HVBS中均发现左旋多巴(L-Dopa),而在EVBS95中未发现。由于EVBS95提取液中出现油状物质(EVBS95油),采用气相色谱-质谱联用技术检测了棕榈酸、亚油酸、油酸和硬脂酸4种脂肪酸。EVBS70、EVBS95和MVBS95对蜡样芽孢杆菌、金黄色葡萄球菌、大肠杆菌、铜绿假单胞菌、肺炎克雷伯菌和伤寒沙门氏菌均有抑菌活性。此外,以1,1-二苯基-2-苦酰肼为自由基的自由基清除实验表明,所有提取物对DPPH的活性都高于市售的左旋多巴、没食子酸、槲皮素和儿茶素。此外,所有提取物对A549、HepG2和SW480细胞系均表现出剂量依赖性的细胞毒作用,其中EVBS70的细胞毒作用最强。除HVBS外,其余提取物对SW480的IC50值均在105 ~ 205 μg/ml之间。本研究提供了酒精提取物对各种药物活性的额外影响,其中泰国丝绒豆是一种有前景的药用和工业用途的经济植物。
Evaluation of Alcoholic Extracts of Mucuna pruriens (L.) DC. Var. utilis for Antibacterial, Antioxidant and Cytotoxic Activities toward Human Cancer Cell Lines
Mucuna pruriens L. DC. Var. utilis (velvet bean), a medicinal Indian plant that has been longterm applied for the treatment of some neurological disorders including Parkinson’s disease. This plant is rich in phytochemical compounds and considered to provide more pharmaceutical medication but those details especially for the velvet bean seeds of the Thai cultivar are still unclear. In this study, the velvet bean seeds extracts were evaluated for their antimicrobial, antioxidant and cytotoxic activities towards three human cancer cell lines. The extractions were performed by ethanol 70% (EVBS70) and 95% (EVBS95), methanol with 70% (MVBS70) and 95% (MVBS95) and hydrochloric acid (HVBS). The quantitative analysis revealed that the highest total phenolic compounds were obtained in MVBS70 with 1,186.37 mg/g gallic acid equivalent. Levodopa (L-Dopa), a major phytochemical in Mucuna seed, was found in EVBS70, MVBS70, MVBS95 and HVBS but not in EVBS95. Since the extraction by EVBS95 appeared an oil-liked substance (EVBS95oil), four fatty acids including palmitic acid, linoleic acid, oleic acid and stearic acid were detected by Gas Chromatography-Mass Spectrometry. The fractions of EVBS70, EVBS95, and MVBS95 exhibited an antibacterial activity for Bacillus cereus, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae and Salmonella typhi. Furthermore, the radical scavenging assay using 1,1-diphenyl-2-picrylhydrazyl as a free radical demonstrated that all extracts presented a higher activity against DPPH than that of the commercially available as L-Dopa, gallic acid, quercetin and catechin. Additionally, all extracts exhibited a dosedependent cytotoxic effect towards A549, HepG2 and SW480 cell lines, while EVBS70 showed the most potent cytotoxic effect. All extracts except HVBS showed the greatest effect on SW480 with IC50 values ranging from 105-205 μg/ml. This study provides additional effects of the alcoholic extracts on various pharmaceutical activities, in which velvet bean in Thailand is a promising economic plant for medical applications and industrial purposes.
期刊介绍:
The Chiang Mai Journal of Science is an international English language peer-reviewed journal which is published in open access electronic format 6 times a year in January, March, May, July, September and November by the Faculty of Science, Chiang Mai University. Manuscripts in most areas of science are welcomed except in areas such as agriculture, engineering and medical science which are outside the scope of the Journal. Currently, we focus on manuscripts in biology, chemistry, physics, materials science and environmental science. Papers in mathematics statistics and computer science are also included but should be of an applied nature rather than purely theoretical. Manuscripts describing experiments on humans or animals are required to provide proof that all experiments have been carried out according to the ethical regulations of the respective institutional and/or governmental authorities and this should be clearly stated in the manuscript itself. The Editor reserves the right to reject manuscripts that fail to do so.