Zahraa K Lawi, Ahmed H Alkhammas, Malek Elerouri, Amara Ibtissem Ben, Mohammed Baqur S Al-Shuhaib
{"title":"端粒酶逆转录酶（TERT）基因的两个共遗传 SNP 与伊拉克肺癌患者有关。","authors":"Zahraa K Lawi, Ahmed H Alkhammas, Malek Elerouri, Amara Ibtissem Ben, Mohammed Baqur S Al-Shuhaib","doi":"10.5937/jomb0-41553","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>The telomerase reverse transcriptase (TERT) gene is essential polymorphic loci linked to most malignant tumors. This study assessed the association between the TERT gene and non-small cell lung carcinoma (NSCLC) in Iraq.</p><p><strong>Methods: </strong>Genomic DNA samples were extracted from a total of 200 samples of blood. Four specific PCR fragments were designed to amplify four high-frequency rs2735940, rs2736098, rs2736100, and rs10069690 SNPs within the TERT gene. Single-strand conformation polymorphism (SSCP) followed by sequencing reactions were used for genotyping and validating the amplified fragments.</p>","PeriodicalId":53750,"journal":{"name":"Fourth Genre-Explorations in Nonfiction","volume":"2 1","pages":"694-705"},"PeriodicalIF":0.1000,"publicationDate":"2023-10-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10710799/pdf/","citationCount":"0","resultStr":"{\"title\":\"Two co-inherited SNPs of the telomerase reverse transcriptase (TERT) gene are associated with Iraqi patients with lung cancer.\",\"authors\":\"Zahraa K Lawi, Ahmed H Alkhammas, Malek Elerouri, Amara Ibtissem Ben, Mohammed Baqur S Al-Shuhaib\",\"doi\":\"10.5937/jomb0-41553\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>The telomerase reverse transcriptase (TERT) gene is essential polymorphic loci linked to most malignant tumors. This study assessed the association between the TERT gene and non-small cell lung carcinoma (NSCLC) in Iraq.</p><p><strong>Methods: </strong>Genomic DNA samples were extracted from a total of 200 samples of blood. Four specific PCR fragments were designed to amplify four high-frequency rs2735940, rs2736098, rs2736100, and rs10069690 SNPs within the TERT gene. Single-strand conformation polymorphism (SSCP) followed by sequencing reactions were used for genotyping and validating the amplified fragments.</p>\",\"PeriodicalId\":53750,\"journal\":{\"name\":\"Fourth Genre-Explorations in Nonfiction\",\"volume\":\"2 1\",\"pages\":\"694-705\"},\"PeriodicalIF\":0.1000,\"publicationDate\":\"2023-10-27\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10710799/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Fourth Genre-Explorations in Nonfiction\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.5937/jomb0-41553\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"0\",\"JCRName\":\"LITERARY REVIEWS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Fourth Genre-Explorations in Nonfiction","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.5937/jomb0-41553","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"0","JCRName":"LITERARY REVIEWS","Score":null,"Total":0}
Two co-inherited SNPs of the telomerase reverse transcriptase (TERT) gene are associated with Iraqi patients with lung cancer.
Background: The telomerase reverse transcriptase (TERT) gene is essential polymorphic loci linked to most malignant tumors. This study assessed the association between the TERT gene and non-small cell lung carcinoma (NSCLC) in Iraq.
Methods: Genomic DNA samples were extracted from a total of 200 samples of blood. Four specific PCR fragments were designed to amplify four high-frequency rs2735940, rs2736098, rs2736100, and rs10069690 SNPs within the TERT gene. Single-strand conformation polymorphism (SSCP) followed by sequencing reactions were used for genotyping and validating the amplified fragments.
京公网安备 11010802042870号
京ICP备2023020795号-1
分享
求助内容：
应助结果提醒方式：
扫码关注我们
