{"title":"含黄花蒿挥发油纳米乳的设计:两株临床标本抗氧化及抑菌活性的研究","authors":"","doi":"10.32592/nkums.15.1.10","DOIUrl":null,"url":null,"abstract":"Introduction: The Artemisia plant is known by its scientific name, which is the exclusive species of Kopeh Daghi in Iran. The present study aimed to design a nanoemulsion of the essential oil of Kopeh Daghi and investigate its antimicrobial activity on the gram-negative bacteria Escherichia coli and the gram-positive bacteria Staphylococcus aureus.\nMethod: At first, the essential oil extraction was performed using a Clevenger. Nanoemulsion was prepared by a spontaneous method using Tween 80 as oil in water. The size of nanoparticles was determined using the dynamic light scattering method. The antioxidant and antibacterial effects of Nanoemulsion at a concentration of 15% of essential oil were investigated.\nResults: In the current research, 38 compounds were identified in the species of Artemisia kopetdaghensis, of which Davanone, with 28.89%, was the most effective substance. The results of the antioxidant test demonstrated that pure essential oil (IC₅₀= 7.05 mg/ml) and Nanoemulsion containing essential oil (IC₅₀= 46.51 mg/ml) had the highest antioxidant activity, respectively. Furthermore, regarding the inhibition of bacterial growth, Nanoemulsion was the most effective in Staphylococcus aureus with a non-growth zone diameter of 8 mm. In Escherichia coli, pure essential oil was the most effective, with a non-growth zone diameter of 9 mm.\nConclusion: In general, the antibacterial properties of 15% nanoemulsion were better compared to 15% essential oil. Moreover, Nanoemulsion containing the medicinal plant essential oil had more antioxidant properties compared to 15% essential oil","PeriodicalId":16423,"journal":{"name":"journal of north khorasan university of medical sciences","volume":"34 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2022-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Design of Nanoemulsions Containing Essential Oil of Artemisia Kopetdaghensis: Investigation of Antioxidant Properties and Antimicrobial Activity on Two Isolates from Clinical Specimens\",\"authors\":\"\",\"doi\":\"10.32592/nkums.15.1.10\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Introduction: The Artemisia plant is known by its scientific name, which is the exclusive species of Kopeh Daghi in Iran. The present study aimed to design a nanoemulsion of the essential oil of Kopeh Daghi and investigate its antimicrobial activity on the gram-negative bacteria Escherichia coli and the gram-positive bacteria Staphylococcus aureus.\\nMethod: At first, the essential oil extraction was performed using a Clevenger. Nanoemulsion was prepared by a spontaneous method using Tween 80 as oil in water. The size of nanoparticles was determined using the dynamic light scattering method. The antioxidant and antibacterial effects of Nanoemulsion at a concentration of 15% of essential oil were investigated.\\nResults: In the current research, 38 compounds were identified in the species of Artemisia kopetdaghensis, of which Davanone, with 28.89%, was the most effective substance. The results of the antioxidant test demonstrated that pure essential oil (IC₅₀= 7.05 mg/ml) and Nanoemulsion containing essential oil (IC₅₀= 46.51 mg/ml) had the highest antioxidant activity, respectively. Furthermore, regarding the inhibition of bacterial growth, Nanoemulsion was the most effective in Staphylococcus aureus with a non-growth zone diameter of 8 mm. In Escherichia coli, pure essential oil was the most effective, with a non-growth zone diameter of 9 mm.\\nConclusion: In general, the antibacterial properties of 15% nanoemulsion were better compared to 15% essential oil. Moreover, Nanoemulsion containing the medicinal plant essential oil had more antioxidant properties compared to 15% essential oil\",\"PeriodicalId\":16423,\"journal\":{\"name\":\"journal of north khorasan university of medical sciences\",\"volume\":\"34 1\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2022-12-17\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"journal of north khorasan university of medical sciences\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.32592/nkums.15.1.10\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"journal of north khorasan university of medical sciences","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.32592/nkums.15.1.10","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Design of Nanoemulsions Containing Essential Oil of Artemisia Kopetdaghensis: Investigation of Antioxidant Properties and Antimicrobial Activity on Two Isolates from Clinical Specimens
Introduction: The Artemisia plant is known by its scientific name, which is the exclusive species of Kopeh Daghi in Iran. The present study aimed to design a nanoemulsion of the essential oil of Kopeh Daghi and investigate its antimicrobial activity on the gram-negative bacteria Escherichia coli and the gram-positive bacteria Staphylococcus aureus.
Method: At first, the essential oil extraction was performed using a Clevenger. Nanoemulsion was prepared by a spontaneous method using Tween 80 as oil in water. The size of nanoparticles was determined using the dynamic light scattering method. The antioxidant and antibacterial effects of Nanoemulsion at a concentration of 15% of essential oil were investigated.
Results: In the current research, 38 compounds were identified in the species of Artemisia kopetdaghensis, of which Davanone, with 28.89%, was the most effective substance. The results of the antioxidant test demonstrated that pure essential oil (IC₅₀= 7.05 mg/ml) and Nanoemulsion containing essential oil (IC₅₀= 46.51 mg/ml) had the highest antioxidant activity, respectively. Furthermore, regarding the inhibition of bacterial growth, Nanoemulsion was the most effective in Staphylococcus aureus with a non-growth zone diameter of 8 mm. In Escherichia coli, pure essential oil was the most effective, with a non-growth zone diameter of 9 mm.
Conclusion: In general, the antibacterial properties of 15% nanoemulsion were better compared to 15% essential oil. Moreover, Nanoemulsion containing the medicinal plant essential oil had more antioxidant properties compared to 15% essential oil