利用一些植物的叶和茎提取物绿色合成纳米银(AgNPs)、表征及其抗菌活性

F. Mohammadi, M. Yousefi, R. Ghahremanzadeh
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引用次数: 23

摘要

绿色合成纳米银颗粒,以沙棘阿魏、豆荚阿魏、灰质teucium poliium venetum叶茎提取物为还原剂和稳定剂。这种合成显示出吸引人的特征,例如;采用廉价、易得的植物提取物,无毒、环保的生物材料,操作简单。AgNO3溶液培养后,提取物的颜色逐渐变为黄褐色,随着培养时间的延长,提取物的颜色逐渐增强。采用紫外可见吸收光谱、x射线衍射(XRD)光谱、动态光散射(DLS)和透射电子显微镜(TEM)对纳米颗粒进行了表征。通过透射电镜观察,合成的银纳米颗粒一般为球形,大小在5 ~ 30 nm之间。生物合成银纳米颗粒(AgNPs)对金黄色葡萄球菌和大肠杆菌两种临床菌株具有良好的抑菌活性。
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Green synthesis, characterization and antimicrobial activity of silver nanoparticles (AgNPs) using leaves and stems extract of some plants
Green synthesis of silver nanoparticles using the aqueous extract of Ferula gumosa, Ferula latisecta, Teucrium polium and Trachomitum venetum leaves and stems extract as the reducing and stabilizing agents. This synthesis shows attractive characteristics such as; the use of inexpensive and available plant extracts, non-toxicity, eco-friendly biological materials, and operational simplicity. The extracts incubated with AgNO3 solution showed gradual change in color of the extracts to yellowish brown, with intensity increasing during the period of incubation. The nanoparticles were characterized by UV–visible absorption spectroscopy, X-ray diffraction (XRD) spectroscopy, Dynamic Light Scattering (DLS), and Transmission Electron Microscope (TEM). The silver nanoparticles synthesized were generally found to be spherical in shape with variable size ranging from 5 to 30 nm, as evident by TEM. The biosynthesized silver nanoparticles (AgNPs) showed good antibacterial activity against clinical strains of two bacteria Staphylococcus aureus and Escherichia coli.
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