{"title":"中药消二结标颗粒中三种有效成分的HPLC测定方法","authors":"Guangcai Qi, Ajing Liu, Z. Liu, Zijin Bai","doi":"10.1109/ICBEB.2012.34","DOIUrl":null,"url":null,"abstract":"A simple, rapid and reproducible HPLC method was used for the simultaneous determination of chlorogenic acid, baicalin and forsythin in Xiao'erjiebiao Granules, at the same time, response surface methodology (RSM) was devoted to forecast the best extraction technology. The HPLC System included an Agilent ZORBAX SB-C18 column ( 250 mm × 4.6 mm, 5μm ), acetonitrile (A) - 0.4% ethylic acid (B) as mobile phase with gradient elution at a flow rate of 1.0 mL.min-1 and detection wavelength of 327 nm for chlorogenic acid, 280 nm for baicalin and forsythin, respectively. The good linearity was achieved successfully with R2 <; 0.9995 in its standard curve aspects. Recoveries measured at the same concentrations (n = 6) were in the range of 96.4-97.6%. The established assay is accurate and practical, which could be utilized as a quality evaluation and control for Xiao'erjie biao Granules.","PeriodicalId":6374,"journal":{"name":"2012 International Conference on Biomedical Engineering and Biotechnology","volume":"26 1","pages":"16-20"},"PeriodicalIF":0.0000,"publicationDate":"2012-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"A Novel HPLC Method for Determining Three Effective Components in Chinese Medicine 'Xiao' Erjiebiao Granules\",\"authors\":\"Guangcai Qi, Ajing Liu, Z. Liu, Zijin Bai\",\"doi\":\"10.1109/ICBEB.2012.34\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"A simple, rapid and reproducible HPLC method was used for the simultaneous determination of chlorogenic acid, baicalin and forsythin in Xiao'erjiebiao Granules, at the same time, response surface methodology (RSM) was devoted to forecast the best extraction technology. The HPLC System included an Agilent ZORBAX SB-C18 column ( 250 mm × 4.6 mm, 5μm ), acetonitrile (A) - 0.4% ethylic acid (B) as mobile phase with gradient elution at a flow rate of 1.0 mL.min-1 and detection wavelength of 327 nm for chlorogenic acid, 280 nm for baicalin and forsythin, respectively. The good linearity was achieved successfully with R2 <; 0.9995 in its standard curve aspects. Recoveries measured at the same concentrations (n = 6) were in the range of 96.4-97.6%. The established assay is accurate and practical, which could be utilized as a quality evaluation and control for Xiao'erjie biao Granules.\",\"PeriodicalId\":6374,\"journal\":{\"name\":\"2012 International Conference on Biomedical Engineering and Biotechnology\",\"volume\":\"26 1\",\"pages\":\"16-20\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2012-05-28\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"2012 International Conference on Biomedical Engineering and Biotechnology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1109/ICBEB.2012.34\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"2012 International Conference on Biomedical Engineering and Biotechnology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1109/ICBEB.2012.34","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
摘要
采用简便、快速、重现性好的高效液相色谱法同时测定消二解表颗粒中绿原酸、黄芩苷和连翘苷的含量，并采用响应面法预测最佳提取工艺。高效液相色谱系统采用Agilent ZORBAX SB-C18色谱柱(250 mm × 4.6 mm, 5μm)，乙腈(A) - 0.4%乙酸(B)为流动相，梯度洗脱，流速1.0 mL.min-1，绿原酸检测波长327 nm，黄芩苷检测波长280 nm，连素检测波长280 nm。在R2 <;在标准曲线方面为0.9995。在相同浓度(n = 6)下，加样回收率为96.4 ~ 97.6%。所建立的方法准确、实用，可作为消儿解标颗粒的质量评价和控制方法。
A Novel HPLC Method for Determining Three Effective Components in Chinese Medicine 'Xiao' Erjiebiao Granules
A simple, rapid and reproducible HPLC method was used for the simultaneous determination of chlorogenic acid, baicalin and forsythin in Xiao'erjiebiao Granules, at the same time, response surface methodology (RSM) was devoted to forecast the best extraction technology. The HPLC System included an Agilent ZORBAX SB-C18 column ( 250 mm × 4.6 mm, 5μm ), acetonitrile (A) - 0.4% ethylic acid (B) as mobile phase with gradient elution at a flow rate of 1.0 mL.min-1 and detection wavelength of 327 nm for chlorogenic acid, 280 nm for baicalin and forsythin, respectively. The good linearity was achieved successfully with R2 <; 0.9995 in its standard curve aspects. Recoveries measured at the same concentrations (n = 6) were in the range of 96.4-97.6%. The established assay is accurate and practical, which could be utilized as a quality evaluation and control for Xiao'erjie biao Granules.
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