商业竞争性elisa法检测哨鸡西尼罗病毒抗体的有效性

S. Peper, Milton Sterling
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引用次数: 0

摘要

哨兵鸡的使用是帮助虫媒病毒监测工作的有力工具。这类监测的结果用于帮助指导减少病媒物种的工作。许多蚊子控制项目使用外包实验室检测哨鸡样本。本研究评估了市售竞争性酶联免疫吸附测定法(cELISA)在鸡血清中西尼罗病毒抗体内部检测中的应用。与外包实验室结果相比,商业cELISA检测到更多阳性哨兵鸡。与外包实验室结果相比,报告鸡血清内部检测结果的时间更早,从而提高了蚊虫控制规划应对蚊媒疾病的及时性。
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EFFECTIVENESS OF A COMMERCIAL COMPETITIVE ELISA FOR THE DETECTION OF WEST NILE VIRUS ANTIBODIES IN SENTINEL CHICKENS
The use of sentinel chickens is a powerful tool to aid in arboviral surveillance efforts. Results from this type of surveillance is used to help guide abatement efforts for vector species. Many mosquito control programs use outsourced laboratories for testing sentinel chicken samples. This study evaluated the use of a commercially available competitive enzyme-linked immunosorbent assay (cELISA) for in-house testing for West Nile virus antibodies from chicken serum. The commercial cELISA detected more positive sentinel chickens compared to the outsourced laboratory results. Results obtained from the in-house testing of chicken serum were reported sooner compared to the outsourced laboratory results, enhancing mosquito control programs timeliness for response to mosquito-borne disease.
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CAPTURING TRENDS IN ARBOVIRAL SURVEILLANCE: COMPARING TRADITIONAL REVERSE TRANSCRIPTION PCR AND QUANTITATIVE REVERSE TRANSCRIPTION PCR ASSAYS TEMPERATURE AND PHOTOPERIOD EFFECT ON DURATION OF GONOTROPHIC DEVELOPMENT AND FECUNDITY OF A LABORATORY COLONY OF AEDES ALBOPICTUS NOVEL TRAP CONFIGURATION FOR LIVE CAPTURE OF MOSQUITOES CRITICAL REVIEW OF INSECTICIDE RESISTANCE IN US AEDES ALBOPICTUS: RESISTANCE STATUS, UNDERLYING MECHANISMS, AND DIRECTIONS FOR FUTURE RESEARCH INSECTICIDE TOXICITY TO HONEY BEES: LESSONS LEARNED FROM STUDIES BY THE UNIVERSITY OF FLORIDA URBAN ENTOMOLOGY LABORATORY
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