{"title":"兔鱼(Siganus canaliculatus)肠道淀粉酶的动力学性质","authors":"Uma Sabapathy, L.H. Teo","doi":"10.1016/0305-0491(94)90150-3","DOIUrl":null,"url":null,"abstract":"<div><p>The optimum pH of the amylase was 6.5 at 30, 37, 40 and 50°C. At pH 6.5, the amylase had an optimum temperature of 25°C and activation energy (<em>E</em><sub>a</sub>) of 7.3 kcal/mol. The thermostability of the enzyme was determined at 45 and 50°C. The <em>K</em><sub>m</sub> values were determined at various temperatures and found to be lowest at 10 and 50°C and highest at 20°C.</p></div>","PeriodicalId":100294,"journal":{"name":"Comparative Biochemistry and Physiology Part B: Comparative Biochemistry","volume":"109 1","pages":"Pages 139-144"},"PeriodicalIF":0.0000,"publicationDate":"1994-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0305-0491(94)90150-3","citationCount":"10","resultStr":"{\"title\":\"Some kinetic properties of amylase from the intestine of the rabbitfish, Siganus canaliculatus (Park)\",\"authors\":\"Uma Sabapathy, L.H. Teo\",\"doi\":\"10.1016/0305-0491(94)90150-3\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>The optimum pH of the amylase was 6.5 at 30, 37, 40 and 50°C. At pH 6.5, the amylase had an optimum temperature of 25°C and activation energy (<em>E</em><sub>a</sub>) of 7.3 kcal/mol. The thermostability of the enzyme was determined at 45 and 50°C. The <em>K</em><sub>m</sub> values were determined at various temperatures and found to be lowest at 10 and 50°C and highest at 20°C.</p></div>\",\"PeriodicalId\":100294,\"journal\":{\"name\":\"Comparative Biochemistry and Physiology Part B: Comparative Biochemistry\",\"volume\":\"109 1\",\"pages\":\"Pages 139-144\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1994-09-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/0305-0491(94)90150-3\",\"citationCount\":\"10\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Comparative Biochemistry and Physiology Part B: Comparative Biochemistry\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/0305049194901503\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Comparative Biochemistry and Physiology Part B: Comparative Biochemistry","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0305049194901503","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Some kinetic properties of amylase from the intestine of the rabbitfish, Siganus canaliculatus (Park)
The optimum pH of the amylase was 6.5 at 30, 37, 40 and 50°C. At pH 6.5, the amylase had an optimum temperature of 25°C and activation energy (Ea) of 7.3 kcal/mol. The thermostability of the enzyme was determined at 45 and 50°C. The Km values were determined at various temperatures and found to be lowest at 10 and 50°C and highest at 20°C.
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