{"title":"废酿酒酵母RNA提取中蛋白质的去除","authors":"Lt, strong gt, 王知力 lt, 郑飞云, 李崎, 顾国贤","doi":"10.3969/J.ISSN.0254-5071.2006.03.009","DOIUrl":null,"url":null,"abstract":"Three methods of removing proteins in RNA were discussed in this paper.The best way was to add 40 U neutral protease per gram dry yeast,and to compare the effects of protein removal to the loss of RNA.The extraction yield of this crude RNA product was 5.75% while the removal ratio of protein was 96.6%.","PeriodicalId":9837,"journal":{"name":"中国酿造","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2006-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Removal of protein in RNA extraction of waste brewing yeast\",\"authors\":\"Lt, strong gt, 王知力 lt, 郑飞云, 李崎, 顾国贤\",\"doi\":\"10.3969/J.ISSN.0254-5071.2006.03.009\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Three methods of removing proteins in RNA were discussed in this paper.The best way was to add 40 U neutral protease per gram dry yeast,and to compare the effects of protein removal to the loss of RNA.The extraction yield of this crude RNA product was 5.75% while the removal ratio of protein was 96.6%.\",\"PeriodicalId\":9837,\"journal\":{\"name\":\"中国酿造\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2006-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"中国酿造\",\"FirstCategoryId\":\"1087\",\"ListUrlMain\":\"https://doi.org/10.3969/J.ISSN.0254-5071.2006.03.009\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"中国酿造","FirstCategoryId":"1087","ListUrlMain":"https://doi.org/10.3969/J.ISSN.0254-5071.2006.03.009","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Removal of protein in RNA extraction of waste brewing yeast
Three methods of removing proteins in RNA were discussed in this paper.The best way was to add 40 U neutral protease per gram dry yeast,and to compare the effects of protein removal to the loss of RNA.The extraction yield of this crude RNA product was 5.75% while the removal ratio of protein was 96.6%.
期刊介绍：
Founded in 1982, China Brewing is a comprehensive academic journal published at home and abroad under the supervision of China Commercial Federation, co-sponsored by China Seasoning Association and Beijing Food Science Research Institute, and is a core journal of natural science in China with wide academic influence in the field of brewing, reflecting the latest research direction, research results and research dynamics of brewing industry, and promoting domestic and international academic exchanges. The main columns of China Brewing are: monographs and reviews, research reports, application technology, analysis and testing, management and marketing.
China Brewing has been published in (SCOPUS), Russian Abstracts Journal (AJ), American Stephens Full Text Database (EBSCO) database, American Ulrich's Guide to Journals (UPD), British Food Science Abstracts (FSTA), British Center for Agricultural and Biological Sciences Research International (CABI), American Chemical Abstracts (CA), Japan Science and Technology Agency (JST) database.
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