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Removal of protein in RNA extraction of waste brewing yeast 废酿酒酵母RNA提取中蛋白质的去除
Pub Date : 2006-01-01 DOI: 10.3969/J.ISSN.0254-5071.2006.03.009
Lt, strong gt, 王知力 lt, 郑飞云, 李崎, 顾国贤
Three methods of removing proteins in RNA were discussed in this paper.The best way was to add 40 U neutral protease per gram dry yeast,and to compare the effects of protein removal to the loss of RNA.The extraction yield of this crude RNA product was 5.75% while the removal ratio of protein was 96.6%.
本文讨论了三种去除RNA中蛋白质的方法。最好的方法是每克干酵母添加40 U中性蛋白酶,并比较蛋白质去除与RNA损失的影响。粗RNA的提取率为5.75%,蛋白质的去除率为96.6%。
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