{"title":"两种表型鉴定试剂盒和经典PCR方法鉴定养鱼场嗜水气单胞菌的效果比较","authors":"Şerafettin Balcı, Z. Ipek, A. Er, Ş. Kayış","doi":"10.3153/ar23013","DOIUrl":null,"url":null,"abstract":"In recent years, phenotypic identification kits have been reported to give incorrect results in identifying Aeromonas species, whereas molecular identification is quite reliable. In this context, Aeromonas hydrophila strains, isolated from fish farms (9 strains), identified by polymerase chain reaction (PCR) method, and ATCC strain bacteria were used in the present study for the determination of the usability of API 20NE and Microgen GN-ID A + B panel test kits. All strains were determined as A. hydrophila in molecular methods. After phenotypic identification, a 100% accuracy rate was obtained for A. hydrophila with API 20NE. In the Microgen GN-ID A + B, these rates were 60% for the strains used in this study. Phenotypic identification for the ATCC strain in both kits was correct. This study showed that the API 20NE test kit had high validation for the rapid and correct identification of fish pathogenic A. hydrophila.","PeriodicalId":13619,"journal":{"name":"International Aquatic Research","volume":"32 1","pages":""},"PeriodicalIF":1.4000,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Comparison of the efficacy of two phenotypic identification kits and classic PCR methods to identify Aeromonas hydrophila isolated from fish farms\",\"authors\":\"Şerafettin Balcı, Z. Ipek, A. Er, Ş. Kayış\",\"doi\":\"10.3153/ar23013\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"In recent years, phenotypic identification kits have been reported to give incorrect results in identifying Aeromonas species, whereas molecular identification is quite reliable. In this context, Aeromonas hydrophila strains, isolated from fish farms (9 strains), identified by polymerase chain reaction (PCR) method, and ATCC strain bacteria were used in the present study for the determination of the usability of API 20NE and Microgen GN-ID A + B panel test kits. All strains were determined as A. hydrophila in molecular methods. After phenotypic identification, a 100% accuracy rate was obtained for A. hydrophila with API 20NE. In the Microgen GN-ID A + B, these rates were 60% for the strains used in this study. Phenotypic identification for the ATCC strain in both kits was correct. This study showed that the API 20NE test kit had high validation for the rapid and correct identification of fish pathogenic A. hydrophila.\",\"PeriodicalId\":13619,\"journal\":{\"name\":\"International Aquatic Research\",\"volume\":\"32 1\",\"pages\":\"\"},\"PeriodicalIF\":1.4000,\"publicationDate\":\"2023-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International Aquatic Research\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.3153/ar23013\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"MARINE & FRESHWATER BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Aquatic Research","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.3153/ar23013","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"MARINE & FRESHWATER BIOLOGY","Score":null,"Total":0}
引用次数: 0
摘要
近年来，据报道，表型鉴定试剂盒在鉴定气单胞菌种类时给出了不正确的结果，而分子鉴定是相当可靠的。在此背景下，本研究采用聚合酶链反应(PCR)方法鉴定了从养鱼场分离的嗜水气单胞菌菌株(9株)和ATCC菌株，以确定API 20NE和Microgen GN-ID A + B面板检测试剂盒的可用性。所有菌株经分子鉴定均为嗜水单胞菌。表型鉴定后，API 20NE对嗜水单胞菌的检测准确率达到100%。在Microgen GN-ID A + B中，本研究中使用的菌株的这些比率为60%。两种试剂盒对ATCC菌株的表型鉴定均正确。本研究表明，API 20NE检测试剂盒对鱼类致病性嗜水单胞菌的快速、正确鉴定具有较高的验证性。
Comparison of the efficacy of two phenotypic identification kits and classic PCR methods to identify Aeromonas hydrophila isolated from fish farms
In recent years, phenotypic identification kits have been reported to give incorrect results in identifying Aeromonas species, whereas molecular identification is quite reliable. In this context, Aeromonas hydrophila strains, isolated from fish farms (9 strains), identified by polymerase chain reaction (PCR) method, and ATCC strain bacteria were used in the present study for the determination of the usability of API 20NE and Microgen GN-ID A + B panel test kits. All strains were determined as A. hydrophila in molecular methods. After phenotypic identification, a 100% accuracy rate was obtained for A. hydrophila with API 20NE. In the Microgen GN-ID A + B, these rates were 60% for the strains used in this study. Phenotypic identification for the ATCC strain in both kits was correct. This study showed that the API 20NE test kit had high validation for the rapid and correct identification of fish pathogenic A. hydrophila.
期刊介绍：
The journal (IAR) is an international journal that publishes original research articles, short communications, and review articles in a broad range of areas relevant to all aspects of aquatic sciences (freshwater and marine). The Journal specifically strives to increase the knowledge of most aspects of applied researches in both cultivated and wild aquatic animals in the world. The journal is fully sponsored, which means it is free of charge for authors. The journal operates a single-blind peer review process. The main research areas in aquatic sciences include: -Aquaculture- Ecology- Food science and technology- Molecular biology- Nutrition- Physiology- Water quality- Climate Change
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