植物原料中多酚类化合物的体外细胞增殖研究

V. Popov, Victoria Aksentyeva
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引用次数: 1

摘要

设计决定食品功能特性的多功能成分的方法之一是由有价值的植物原料组成的复杂食品添加剂。添加剂的质量取决于所用原料是否充足、采集时间和地点以及植物栽培条件。传统的种植方法需要大量的成本来获得决定植物生理材料价值的次生代谢物。研究目的是在实验室无菌条件下通过微克隆细胞增殖从植物原料中获得多酚类化合物。以含有大量多酚的蔓越莓和越橘的叶子和浆果细胞为研究对象。例如,生长在秋明地区南部的蔓越莓中花青素含量为97.8毫克/100克,白花色素含量为459.6毫克/100克,而生长在亚马尔-涅涅茨自治区北极地区的浆果中花青素含量分别为224.7毫克/100克和480.2毫克/100克。在初始阶段,研究人员对物体、仪器和设备进行了消毒。他们通过实验确定了灭菌时间。研究表明,随着灭菌时间的延长,培养基颜色发生变化,细胞毒性增加,在最短的灭菌时间内,病原菌仍留在培养基中。灭菌后,一人在照度为100 mmol量子/m²的荧光灯下进行光培养;每天20小时的光周期;在pH值为5.2-5.4的营养培养基中;体积25ml;富含生长素和细胞分裂素激素。研究人员使用昆都植物激素来调节次生代谢物的合成。他们利用Murashige Skoog和Anderson琼脂培养基寻找最佳的营养培养基,以获得植物细胞中多酚类化合物的最大增加。一个人独立开发了第三种琼脂培养基,考虑到个体方法对更有效的生产者生长的前景。
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Development of the Polyphenol Compounds from Plant Raw Materials by the Cell Micropropagation IN VITRO Method
One of the ways to design polyfunctional ingredients that determine the functional properties of food products are complex food additives consisting of valuable plant raw materials. The additives quality depends on the used raw materials adequacy, time and collection place, and the plant cultivation conditions. The traditional plantation method requires significant costs to obtain secondary metabolites determining physiological plant materials value. The research aim is to obtain polyphenolic compounds from phyto-raw materials by microclonal cell propagation under sterile laboratory conditions. The leaves and berries cells of cowberries and cranberries containing a significant number of polyphenols are the research objects. For example, cranberries growing in the south of the Tyumen region contain anthocyanins 97.8 mg/100 g and leukoanthocyanins 459.6 mg/100 g, and berries growing in the Arctic territories of the Yamalo-Nenets Autonomous Okrug contain 224.7 and 480.2 mg/100 g, respectively. At the initial stage, the researchers sterilized objects, instruments, and equipment. They determined the sterilization duration experimentally. The study revealed that with a longer sterilization duration, there was a change in color and an increase in cell toxicity, with a minimum duration, pathogenic microorganisms remained in the medium. After sterilization, a man cultured the cells in the light under fluorescent lamps with illumination of 100 mmol quanta/m²; photoperiod of 20 h per day; in nutrient media with an acidity of pH 5.2–5.4; 25 ml in volume; enriched with the auxins and cytokinins hormones. The researchers used Kundu phytohormones to regulate the secondary metabolites synthesis. They utilized Murashige Skoog and Anderson agar media to find the optimal nutrient medium in order to obtain the maximum increase in polyphenolic compounds in plant cells. A man developed the third agar media independently, considering the prospects of an individual approach to the more efficient producers growth.
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来源期刊
Agro Food Industry Hi-tech
Agro Food Industry Hi-tech 工程技术-生物工程与应用微生物
CiteScore
0.29
自引率
0.00%
发文量
0
审稿时长
6-12 weeks
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