{"title":"废水中分离菌株NAP a的萘降解能力及分子系统发育","authors":"Chunyang Zhang, Z. Peng","doi":"10.1109/ICBBE.2008.1147","DOIUrl":null,"url":null,"abstract":"In the study, a selective medium was used to enrich and isolate a naphthalene-degrading bacterium NAP_A from the activated sludge of Zhangdian Municipal Wastewater Treatment Plant in Zibo, China. Cultivation and gas chromatography analysis showed NAP_A degraded naphthalene fast under 30degC~35degC and pH7. The 16S rRNA gene of NAP A was cloned and sequenced (EU142847). Basing on this sequence and its related sequences in the GenBank database, a multialignment was conducted then a phylogenetic tree was constructed with DNAMAN software. The results showed that strain NAP_A was located in the lineage of Ochrobactrum genus, sharing the highest homology with O. pseudintermedium (99%), which indicated the strain was a member of O. pseudintermedium. This revealed for the first time that Ochrobactrum species could degrade polycyclic aromatic hydrocarbon, which would guide the biotreatment of such environmental contaminant with the bacterium.","PeriodicalId":6399,"journal":{"name":"2008 2nd International Conference on Bioinformatics and Biomedical Engineering","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2008-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"4","resultStr":"{\"title\":\"Naphthalene Degradation Capability and Molecular Phylogeny of a Bacterial Strain NAP A Isolated from Wastewater\",\"authors\":\"Chunyang Zhang, Z. Peng\",\"doi\":\"10.1109/ICBBE.2008.1147\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"In the study, a selective medium was used to enrich and isolate a naphthalene-degrading bacterium NAP_A from the activated sludge of Zhangdian Municipal Wastewater Treatment Plant in Zibo, China. Cultivation and gas chromatography analysis showed NAP_A degraded naphthalene fast under 30degC~35degC and pH7. The 16S rRNA gene of NAP A was cloned and sequenced (EU142847). Basing on this sequence and its related sequences in the GenBank database, a multialignment was conducted then a phylogenetic tree was constructed with DNAMAN software. The results showed that strain NAP_A was located in the lineage of Ochrobactrum genus, sharing the highest homology with O. pseudintermedium (99%), which indicated the strain was a member of O. pseudintermedium. This revealed for the first time that Ochrobactrum species could degrade polycyclic aromatic hydrocarbon, which would guide the biotreatment of such environmental contaminant with the bacterium.\",\"PeriodicalId\":6399,\"journal\":{\"name\":\"2008 2nd International Conference on Bioinformatics and Biomedical Engineering\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2008-05-16\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"4\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"2008 2nd International Conference on Bioinformatics and Biomedical Engineering\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1109/ICBBE.2008.1147\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"2008 2nd International Conference on Bioinformatics and Biomedical Engineering","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1109/ICBBE.2008.1147","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Naphthalene Degradation Capability and Molecular Phylogeny of a Bacterial Strain NAP A Isolated from Wastewater
In the study, a selective medium was used to enrich and isolate a naphthalene-degrading bacterium NAP_A from the activated sludge of Zhangdian Municipal Wastewater Treatment Plant in Zibo, China. Cultivation and gas chromatography analysis showed NAP_A degraded naphthalene fast under 30degC~35degC and pH7. The 16S rRNA gene of NAP A was cloned and sequenced (EU142847). Basing on this sequence and its related sequences in the GenBank database, a multialignment was conducted then a phylogenetic tree was constructed with DNAMAN software. The results showed that strain NAP_A was located in the lineage of Ochrobactrum genus, sharing the highest homology with O. pseudintermedium (99%), which indicated the strain was a member of O. pseudintermedium. This revealed for the first time that Ochrobactrum species could degrade polycyclic aromatic hydrocarbon, which would guide the biotreatment of such environmental contaminant with the bacterium.
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