对水稻的生物农药作用研究

W. Musa, Suleman Duengo, A. Kilo, Boima Situmeang
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引用次数: 1

摘要

本研究的目的是研究野栗籽提取物活性化合物作为蔬菜农药对水稻土壤害虫的防治效果。这些研究的方法是分离获得纯分离物,用红外分光光度计(IR)和紫外-可见分光光度计进行鉴定,然后进行生物学试验。获得的分离物经检测为植物化学阳性,含有生物碱化合物,表征为无定形晶体。红外分光光度计鉴定得到3423.4 cm-1波长区域的功能-NH波群,波数为1242.1 cm-1的C- n弯曲,波数为2925.8和2854.5 cm-1的C- h伸展,波数为1475 cm-1的C- h弯曲,波数为1540 cm-1,波数为1720.4 cm-1的羰基(C = O)可能是生物碱化合物。紫外-可见分光光度计在209.9 nm波长处测得最大吸收,表明C = O官能团之间存在双键,这可能是由于连续的电子跃迁到n-π *和n-σ *的结果。生物试验结果表明,桔梗种子甲醇提取物可作为蔬菜农药用于水稻病虫害防治,生物试验中使用的最有效浓度为0.1%,对水稻病虫害的防治效果非常好。
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Alkaloid compound from Tombili (Caesalpinia bonduc) as biopesticide agent on rice plants
The purpose of these study is to determine the effect of active compound from tombili seed extract as a vegetable pesticide in the prevention of soil pest in rice plants. The method of these researches is isolation to obtain pure isolates, characterization by using infrared spectrophotometer (IR) and UV-VIS spectrophotometer then following biological test. The isolates obtained were tested positive for phytochemical containing alkaloid compounds and characterized as amorphous crystals. Identification of IR spectrophotometer generate a functioning -NH wave group of the wavelengths region of 3423.4 cm-1, bend C-N at wave number 1242.1 cm-1, C-H stretch at wave number 2925.8 and 2854.5 cm-1, bend C-H at 1475 wave numbers 1540 cm-1, carbonyl group (C = O) at the 1720.4 cm-1 wave number which is probably an alkaloid compound. The UV-VIS spectrophotometer results in a maximum absorption at 209.9 nm wavelengths indicated the presence of double bonds between the C = O functional group suspected as a result of the successive electronic transitions to n-π * and n-σ *. The result of biological test showed that methanol extracts of tombili seed can be used as a vegetable pesticide in pest prevention on rice plants, the most effective concentration used in this biological test is at 0.1% concentration, with very high effectiveness in eradicating pest on rice plants.
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