Oana Hosu, Mihaela Tertiş, Gheorghe Melinte, Robert Săndulescu, Cecilia Cristea
{"title":"基于对氨基苯基乙酸接枝碳电极作为固定平台的粘蛋白4免疫传感器","authors":"Oana Hosu, Mihaela Tertiş, Gheorghe Melinte, Robert Săndulescu, Cecilia Cristea","doi":"10.1016/j.protcy.2017.04.048","DOIUrl":null,"url":null,"abstract":"<div><p>A simple impedimetric label-free immunosensor was developed for the specific and sensitive detection of mucin 4 (MUC 4) protein by using graphite based screen printed electrodes modified with an aryl diazonium salt or compound (<em>p</em>- aminophenylacetic acid) for the immobilization of antibody anti-MUC4 via amidic bond. Electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV) were used in order to characterize and optimize the electrografting process. The parameters involved in each step of the immunosensor design were optimized. The performance of the immunoassay in terms of sensitivity, reproducibility and selectivity was studied.</p></div>","PeriodicalId":101042,"journal":{"name":"Procedia Technology","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.protcy.2017.04.048","citationCount":"1","resultStr":"{\"title\":\"Mucin 4 Immunosensor Based on p-aminophenylacetic Acid Grafting on Carbon Electrodes as Immobilization Platform\",\"authors\":\"Oana Hosu, Mihaela Tertiş, Gheorghe Melinte, Robert Săndulescu, Cecilia Cristea\",\"doi\":\"10.1016/j.protcy.2017.04.048\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>A simple impedimetric label-free immunosensor was developed for the specific and sensitive detection of mucin 4 (MUC 4) protein by using graphite based screen printed electrodes modified with an aryl diazonium salt or compound (<em>p</em>- aminophenylacetic acid) for the immobilization of antibody anti-MUC4 via amidic bond. Electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV) were used in order to characterize and optimize the electrografting process. The parameters involved in each step of the immunosensor design were optimized. The performance of the immunoassay in terms of sensitivity, reproducibility and selectivity was studied.</p></div>\",\"PeriodicalId\":101042,\"journal\":{\"name\":\"Procedia Technology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2017-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/j.protcy.2017.04.048\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Procedia Technology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S221201731730049X\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Procedia Technology","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S221201731730049X","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Mucin 4 Immunosensor Based on p-aminophenylacetic Acid Grafting on Carbon Electrodes as Immobilization Platform
A simple impedimetric label-free immunosensor was developed for the specific and sensitive detection of mucin 4 (MUC 4) protein by using graphite based screen printed electrodes modified with an aryl diazonium salt or compound (p- aminophenylacetic acid) for the immobilization of antibody anti-MUC4 via amidic bond. Electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV) were used in order to characterize and optimize the electrografting process. The parameters involved in each step of the immunosensor design were optimized. The performance of the immunoassay in terms of sensitivity, reproducibility and selectivity was studied.
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