铜绿假单胞菌生物膜形成及部分毒力因子的检测及抗生素的作用

Q4 Biochemistry, Genetics and Molecular Biology Mikrobiolohichnyi zhurnal Pub Date : 2022-11-28 DOI:10.15407/microbiolj84.02.033
J. H. Makhrmash
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引用次数: 0

摘要

目标。铜绿假单胞菌是一种普遍存在的机会致病菌。许多毒力因子如外毒素、外酶基因和生物膜的存在可能在细菌的发病和致病性中起作用。本研究的目的是检测铜绿假单胞菌的生物膜形成、部分生物膜基因以及抗生素对铜绿假单胞菌的作用。方法。所有分离株均采用API 20E和16S rRNA技术进行鉴定。采用微滴板法(MTPM)检测生物膜的形成。采用聚合酶链反应(PCR)技术对pelA、pslA等毒力基因进行检测。结果。64株铜绿假单胞菌被鉴定为铜绿假单胞菌。烧伤感染27例(42.2%),耳部感染17例(26.5%),尿部感染20例(31.3%)。MTPM生物膜检测结果表明,所有铜绿假单胞菌分离株均能产生生物膜,但水平不同。采用PCR技术检测生物膜基因。研究表明,61株(95.30%)和63株(99.32%)分别携带pelA和pslA基因。并进行药敏试验,筛选出10种抗生素。铜绿假单胞菌对头孢噻肟61(95.3%)、卡比西林59(92.2%)、氨苄西林38(59.4%)、哌拉西林/他唑巴坦29(45.3%)、链霉素28(43.8%)、莫西沙星27(42.4%)、替卡西林26(40.6%)、环丙沙星24(37.5%)、庆大霉素20(31.3%)、新霉素13(20.3%)耐药。结论。铜绿假单胞菌在不同水平上产生生物膜。分子技术表明,pslA和pelA基因与铜绿假单胞菌生物膜的形成有关。药敏试验显示,对铜绿假单胞菌最有效的抗生素分别是新霉素、庆大霉素和环丙沙星。
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Detection of Biofilm Formation and Some Virulence Factors in Pseudomonas aeruginosa, and the Effect of Some Antibiotics
Objective. Pseudomonas aeruginosa is a present everywhere and opportunistic bacterium pathogen. The existence of numerous virulence factors i.e. exo-toxin, exo-enzyme genes, and biofi lm may be contributed in the pathogenesis and pathogenicity of the bacterium. The goals of the present work were to detect biofilm formation, some biofilm genes, and the effect of antibiotics against P. aeruginosa. Methods. All isolates were identified using API 20E and 16S rRNA techniques. The microtiter plate method (MTPM) was used to detect biofi lm formation. Th e polymerase chain reaction (PCR) was used to fi nd some virulence genes e.g. pelA, pslA. Results. A total of 64 P. aeruginosa isolates were identified as P. aeruginosa. The majority of infection belonged to burn infections — 27 (42.2%), followed by ear — 17 (26.5%), and urine — 20 (31.3%). The results of biofilm detection using MTPM showed that all P. aeruginosa isolates were able to produce biofilm but at different levels. PCR technique was used to detect biofilm genes. Studies showed that 61 (95.30%) and 63 (99.32%) isolates carried pelA and pslA genes, respectively. Moreover, a susceptibility test was used to select 10 antibiotics. P. aeruginosa isolates were resistant to cefotaxime — 61 (95.3%), carbenicillin — 59 (92.2%), ampicillin — 38 (59.4%), piperacilin/tazobactam — 29 (45.3%), streptomycin — 28 (43.8%), moxifloxacin — 27 (42.4%), ticarcilin — 26 (40.6%), ciprofloxacin — 24 (37.5%), gentamicin — 20 (31.3%), and neomycin — 13 (20.3%). Conclusions. Biofilm is produced by P. aeruginosa at different levels. The molecular technique showed that the pelA and pslA genes are associated with the form of biofilm in P. aeruginosa isolates. The susceptibility tests showed that the most active antibiotics against P. aeruginosa were neomycin, gentamycin, and ciprofloxacin, respectively.
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Mikrobiolohichnyi zhurnal
Mikrobiolohichnyi zhurnal Medicine-Microbiology (medical)
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