Bioactive Compounds from Mango peels (Mangifera indica .Tommy Atkins) And Demonstration of its Cytotoxicity and CCl4 Induced Hepatotoxicity in Rat

Mangifera indica (Tommy Atkins) peels, commonly known as mango, is a pharmacologically, ethnomedically, and phytochemically diverse plant. Peels is a major by-product during processing of mango fruit into pulp. In the present study, Thirteen pure bioactive compounds were isolated from methanolic peels extract. Six of them are new ellagitannins,namely, 1,2,3,4,6 -Penta-O-galloyl-ß-C1-glupyranose (3); 2,3,6-Tri-O-galloyl-(α/β)-C1-glucopyranose(4); 2,3-Di-O-galloyl-(α/β)C1-glucopyranose,Nilocitin(6);3,6-Di-O-galloyl-(α/β)-C1-glucopyranose(8);1,6 -Di-O-galloylβ-C1-glucopyranose (9) ; 1,3-Di-O-galloyl-β-C1-glucose (11), which were analyzed for the first time from M. indica (Tommy Atkins) peels. The ameliorative effect of the methanolic extract of M. indica (Tommy Atkins) peels towards the CCl4-induced hepatotoxicity in male Wistar rats through measuring certain biochemical parameters content in the liver were analyzed. The CCl4-treated rats showed a significant decline in the studied the serum levels of high-density lipoprotein (HDL), albumin (A) as well as the hepatic levels of glutathione (GSH) and activities of catalase (CAT), superoxide dismutase (SOD) , glutathione reductase (GR), elevation in the levels of total lipids (TL), triglycerides (TG), total cholesterol (TC), low-density lipoproteins (LDL), globulin (G), total bilirubin (TBil) , alanine and aspartate aminotransferase and alkaline phosphatase (ALAT and ASAT, ALP) and the hepatic levels of malondialdehyde (MDA). In contrast, the administration of methanol extract, notably improved all the studied parameters. This study showed that CCl4 administration to Wistar rats, at a high dose level, could induce a hepatic injury in addition to certain metabolic alterations. The work was extended to investigate tissue histopathology. Thus, results suggest that the peels extract can be a potential source of an attractive candidate for ameliorating of hepatotoxicity induced by CCl4 through scavenging free radicals, improved liver functions, and normalizing the liver histopathological architecture. Keywors: Mangifera indica (Tommy Atkins) peels, Phenolics, gallotannines, NMR spectroscopy, In Vivo Hepatoprotective activity