{"title":"胰蛋白酶处理的精子对人卵泡卵母细胞的体外受精","authors":"H. Kubo, S. Katayama, Motoyuki Hayashi","doi":"10.1111/J.1447-0756.1981.TB00531.X","DOIUrl":null,"url":null,"abstract":"In vitro fertilization of human follicular oocytes was carried out using trypsin-treated spermatozoa. The oocytes were recovered, from ovaries in situ by culdoscope with a special oocyte recovery apparatus: Using this apparatus, a total of 165 oocytes were recovered by aspirating 324 follicles from 80 patients (average 2.1 oocytes/patient). Degenerated oocytes (32) at the initial evaluation were not included in the experiment and the 61 remaining (45.9%) were matured in vitro. The matured oocytes were then inseminated by trypsin-treated or untreated spermatozoa at concentrations of 6 to 20 times 106 sperm/ml. Eighteen of 31 oocytes (58.1%) inseminated by trypsin-treated spermatozoa were fertilized and three (9.7%) of the fertilized oocytes developed to a blastocyst stage, whereas 6 out of 30 oocytes (20%) which were inseminated with non-treated spermatozoa were fertilized and none of them developed beyond a 16-cell stage. \n \n \n \nTherefore, it appears that trypsin-treatment may increase the fertilizing ability of human spermatozoa.","PeriodicalId":8557,"journal":{"name":"Asia-Oceania journal of obstetrics and gynaecology","volume":"1 1","pages":"251-258"},"PeriodicalIF":0.0000,"publicationDate":"2010-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"In Vitro Fertilization of Human Follicular Oocytes by Trypsin‐Treated Spermatozoa\",\"authors\":\"H. Kubo, S. Katayama, Motoyuki Hayashi\",\"doi\":\"10.1111/J.1447-0756.1981.TB00531.X\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"In vitro fertilization of human follicular oocytes was carried out using trypsin-treated spermatozoa. The oocytes were recovered, from ovaries in situ by culdoscope with a special oocyte recovery apparatus: Using this apparatus, a total of 165 oocytes were recovered by aspirating 324 follicles from 80 patients (average 2.1 oocytes/patient). Degenerated oocytes (32) at the initial evaluation were not included in the experiment and the 61 remaining (45.9%) were matured in vitro. The matured oocytes were then inseminated by trypsin-treated or untreated spermatozoa at concentrations of 6 to 20 times 106 sperm/ml. Eighteen of 31 oocytes (58.1%) inseminated by trypsin-treated spermatozoa were fertilized and three (9.7%) of the fertilized oocytes developed to a blastocyst stage, whereas 6 out of 30 oocytes (20%) which were inseminated with non-treated spermatozoa were fertilized and none of them developed beyond a 16-cell stage. \\n \\n \\n \\nTherefore, it appears that trypsin-treatment may increase the fertilizing ability of human spermatozoa.\",\"PeriodicalId\":8557,\"journal\":{\"name\":\"Asia-Oceania journal of obstetrics and gynaecology\",\"volume\":\"1 1\",\"pages\":\"251-258\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2010-05-24\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Asia-Oceania journal of obstetrics and gynaecology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1111/J.1447-0756.1981.TB00531.X\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Asia-Oceania journal of obstetrics and gynaecology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1111/J.1447-0756.1981.TB00531.X","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
In Vitro Fertilization of Human Follicular Oocytes by Trypsin‐Treated Spermatozoa
In vitro fertilization of human follicular oocytes was carried out using trypsin-treated spermatozoa. The oocytes were recovered, from ovaries in situ by culdoscope with a special oocyte recovery apparatus: Using this apparatus, a total of 165 oocytes were recovered by aspirating 324 follicles from 80 patients (average 2.1 oocytes/patient). Degenerated oocytes (32) at the initial evaluation were not included in the experiment and the 61 remaining (45.9%) were matured in vitro. The matured oocytes were then inseminated by trypsin-treated or untreated spermatozoa at concentrations of 6 to 20 times 106 sperm/ml. Eighteen of 31 oocytes (58.1%) inseminated by trypsin-treated spermatozoa were fertilized and three (9.7%) of the fertilized oocytes developed to a blastocyst stage, whereas 6 out of 30 oocytes (20%) which were inseminated with non-treated spermatozoa were fertilized and none of them developed beyond a 16-cell stage.
Therefore, it appears that trypsin-treatment may increase the fertilizing ability of human spermatozoa.