胰蛋白酶处理的精子对人卵泡卵母细胞的体外受精

H. Kubo, S. Katayama, Motoyuki Hayashi
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引用次数: 0

摘要

用胰蛋白酶处理过的精子进行了人卵泡卵母细胞的体外受精。使用专用的卵母细胞恢复装置,在显微镜下从卵巢原位恢复卵母细胞。使用该装置,从80例患者中抽取324个卵泡(平均2.1个卵母细胞/例),共回收165个卵母细胞。初步评估时退行性卵母细胞(32个)未纳入实验,其余61个(45.9%)在体外成熟。然后将成熟的卵母细胞与胰蛋白酶处理或未处理的精子以6至20倍106精子/ml的浓度进行受精。经胰蛋白酶处理的31个卵母细胞中有18个(58.1%)受精卵,其中3个(9.7%)受精卵发育到囊胚期,而未经胰蛋白酶处理的30个卵母细胞中有6个(20%)受精卵未发育到16细胞期。因此,胰蛋白酶处理似乎可以提高人类精子的受精能力。
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In Vitro Fertilization of Human Follicular Oocytes by Trypsin‐Treated Spermatozoa
In vitro fertilization of human follicular oocytes was carried out using trypsin-treated spermatozoa. The oocytes were recovered, from ovaries in situ by culdoscope with a special oocyte recovery apparatus: Using this apparatus, a total of 165 oocytes were recovered by aspirating 324 follicles from 80 patients (average 2.1 oocytes/patient). Degenerated oocytes (32) at the initial evaluation were not included in the experiment and the 61 remaining (45.9%) were matured in vitro. The matured oocytes were then inseminated by trypsin-treated or untreated spermatozoa at concentrations of 6 to 20 times 106 sperm/ml. Eighteen of 31 oocytes (58.1%) inseminated by trypsin-treated spermatozoa were fertilized and three (9.7%) of the fertilized oocytes developed to a blastocyst stage, whereas 6 out of 30 oocytes (20%) which were inseminated with non-treated spermatozoa were fertilized and none of them developed beyond a 16-cell stage. Therefore, it appears that trypsin-treatment may increase the fertilizing ability of human spermatozoa.
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