椰子、棕榈果和椰子酒液体胚乳中银纳米粒子的绿色合成、表征及其抗菌活性

Nisha P. Aravind, Athira Rajeev, Jobin Mathew
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摘要

纳米粒子是直径在1nm到100nm之间的分子,可以通过物理、化学和生物方法合成。绿色合成包括从细菌、真菌、藻类、植物和植物提取物中生产纳米颗粒。植物提取物及其化学成分可以从盐溶液中捕获目标金属,并通过细胞催化剂将金属颗粒转化为纳米颗粒。以椰子液体胚乳、椰子水(Cocos nucifera)、棕榈果实液体胚乳(Borassus flabellifer)和椰子酒为还原剂,进行了纳米银的绿色合成研究。用椰子水对银离子的生物还原是通过其颜色变化来确定的。在纳米银的合成过程中,溶液的颜色由乳白色变为棕色。利用傅里叶变换红外光谱和紫外可见光谱对合成的银纳米粒子进行了表征。在250和350 nm处获得的峰证实了合成银纳米颗粒的存在。采用圆盘扩散法研究了合成的纳米颗粒对大肠杆菌、金黄色葡萄球菌和化脓性链球菌的抑菌活性。近年来,绿色合成技术以其环保、经济、可行、无毒等特点而备受瞩目。
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Green synthesis and Characterisation of Silver Nanoparticles from liquid endosperm of Coconut, Palm fruit and Coconut toddy and it’s Antibacterial Activity
Nanoparticles are molecules in the range of 1 nm to 100 nm and they can be synthesized by physical, chemical and biological methods. Green synthesis involves the production of nanoparticles from bacteria, fungi, algae, plants and plant extracts. Plant extracts with their chemical constituents can capture the target metals from their salt solution and transform the metal particles into nanoparticles with the cellular catalysts. The present study focuses on the green synthesis of silver nanoparticles using the liquid endosperm of coconut, the coconut water (Cocos nucifera), Liquid endosperm of palm fruit (Borassus flabellifer) and coconut Toddy as the reducing agent. The bio-reduction of aqueous silver ions by coconut water is identified by its colour change. During the synthesis of silver nanoparticles, the colour of the solution changed from milky white to brown. The characterization of synthesized AgNPs (Silver Nanoparticles) was done by Fourier Transform Infrared Spectroscopy and UV-Vis Spectroscopy. The peaks obtained at 250 and 350 nm confirmed the presence of synthesized Silver Nanoparticles. The antibacterial activity of the synthesized nanoparticles was studied on Escherichia coli, Staphylococcus aureus and Streptococcus pyogenes using disc diffusion method. The recent approach of green synthesis is quite impressive due to its eco- friendly, economical, feasible and non- toxic nature.
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