两个大麦品种盐度变化的比较

A. Temel
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引用次数: 2

摘要

比较了两种大麦品种(Hordeum vulgare cv.)的体外盐度效应。Bornova-92和Hilal)。成熟胚分别在添加0、50和100 mM NaCl的Murashige和Skoog培养基上培养20 d。nacl处理降低了两个品种的最大茎长、总可溶性蛋白和DNA含量,但降低了Hilal的最大根长。鲜重、干重和水分含量变化无统计学意义。抑制作用在Hilal中更为明显。盐度在两个品种中均未引起遗传毒性效应,但对Bornova-92的蛋白质模式有轻微影响。然而,盐度改变了Bornova-92中从CCG到CG的胞嘧啶甲基化模式,在Hilal中从CG到CCG。Bornova-92和Hilal可能分别被认为是耐盐和盐敏感,Bornova-92的相对耐盐性可能是由于胞嘧啶甲基化模式和/或蛋白质合成的调节。
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Comparison of Salinity-Induced Changes in Two Cultivars of Barley
In vitro effects of salinity were compared in two cultivars of barley (Hordeum vulgare cv. Bornova-92 and Hilal). Mature embryos were cultured on Murashige and Skoog media supplemented with 0, 50 and 100 mM NaCl for 20 days. NaCl-treatment decreased maximum shoot length, total soluble protein and DNA contents in both cultivars but decreased maximum root length in Hilal. Changes in fresh and dry weight and water content were not statistically-significant. Inhibitory effects were more dramatic in Hilal. Salinity did not cause genotoxic effects in both cultivars yet slightly affected protein patterns in Bornova-92. However, salinity altered cytosine methylation patterns from CCG to CG in Bornova-92, from CG to CCG in Hilal. Bornova-92 and Hilal may be regarded as salt-tolerant and salt-susceptible, respectively and the relative salt-tolerance of Bornova-92 may be due to cytosine methylation patterns and/or regulation of protein synthesis.
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