二氧化铈是一种具有双重人格的纳米材料,可以增加和减少炎症和氧化应激

IF 4.2 2区 医学 Q2 MEDICINE, RESEARCH & EXPERIMENTAL Nanomedicine : nanotechnology, biology, and medicine Pub Date : 2022-07-01 DOI:10.1016/j.nano.2022.102565
Robert A. Yokel PhD , Marsha L. Ensor MSc , Hemendra J. Vekaria PhD , Patrick G. Sullivan PhD , David J. Feola PharmD, PhD , Arnold Stromberg PhD , Michael T. Tseng PhD , Douglas A. Harrison PhD
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引用次数: 3

摘要

假设催化剂纳米粒可以从基础状态增加炎症/氧化应激,从升高状态减少炎症/氧化应激。巨噬细胞清除纳米细胞。为了验证这一假设,我们将M0(非极化)、M1-(经典活化、促炎)和m2样(选择性活化、调节性表型)的RAW 264.7巨噬细胞暴露在纳米细胞中。通过IL-1β水平、精氨酸酶活性和RT-qPCR量化炎症反应,通过mito和糖酵解应激试验(MST和GST)量化代谢变化和氧化应激。形态学通过光镜、巨噬细胞表型标记表达和一种新的三维免疫组织化学方法来确定。纳米粒阻断了IL-1β和精氨酸酶的作用,增加了M0细胞向M2表型的OCR和GST,并改变了多个M1和M2样细胞的端点向M0水平。m1样细胞体积更大,圆度更小。m2样细胞体积大于M0巨噬细胞。结果总体上与假设一致。
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Cerium dioxide, a Jekyll and Hyde nanomaterial, can increase basal and decrease elevated inflammation and oxidative stress

It was hypothesized that the catalyst nanoceria can increase inflammation/oxidative stress from the basal and reduce it from the elevated state. Macrophages clear nanoceria. To test the hypothesis, M0 (non-polarized), M1- (classically activated, pro-inflammatory), and M2-like (alternatively activated, regulatory phenotype) RAW 264.7 macrophages were nanoceria exposed. Inflammatory responses were quantified by IL-1β level, arginase activity, and RT-qPCR and metabolic changes and oxidative stress by the mito and glycolysis stress tests (MST and GST). Morphology was determined by light microscopy, macrophage phenotype marker expression, and a novel three-dimensional immunohistochemical method. Nanoceria blocked IL-1β and arginase effects, increased M0 cell OCR and GST toward the M2 phenotype and altered multiple M1- and M2-like cell endpoints toward the M0 level. M1-like cells had greater volume and less circularity/roundness. M2-like cells had greater volume than M0 macrophages. The results are overall consistent with the hypothesis.

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来源期刊
CiteScore
11.10
自引率
0.00%
发文量
133
审稿时长
42 days
期刊介绍: The mission of Nanomedicine: Nanotechnology, Biology, and Medicine (Nanomedicine: NBM) is to promote the emerging interdisciplinary field of nanomedicine. Nanomedicine: NBM is an international, peer-reviewed journal presenting novel, significant, and interdisciplinary theoretical and experimental results related to nanoscience and nanotechnology in the life and health sciences. Content includes basic, translational, and clinical research addressing diagnosis, treatment, monitoring, prediction, and prevention of diseases.
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