Assessment of electron beam irradiation induced proteomic changes and its effect on the development of silkworm, Bombyx mori (Bombycidae: Lepidoptera)

The present study has been designed to determine the hemolymph protein changes induced by EBI using Microtron (ranges from 20 to 100 Gy) on the 5^th instar larvae of silkworm, Bombyx mori using SDS–PAGE. The EBI did not caused any impact on 5^th instar larval hemolymph proteins, however a significant reduction of pupal hemolymph proteins such as lipophorin (250 kDa), vitellogenin (180 kDa), storage protein (76–80 kDa) and a 30 kDa protein was observed through SDS–PAGE and densitometry analysis. These proteins are known to play a crucial role in various developmental processes including transport of lipids, as amino acid reservoir for providing precursors for egg and cuticle formation and immunity of B. mori. Further, a decrease of antioxidant enzymes such as Superoxide Dismutase (SOD) and Catalase (CAT) in the EBI treated larval and pupal hemolymph was observed. In addition, a negative influence on growth characteristics and appearance of pupal deformity was noted. This may be due to damage in hemolymph proteins, when the larvae were exposed to EBI at >80 Gy.