Bioactive Composition, Antioxidant, And Cytotoxic Activities of Rheum Ribes Extracts

The aim of the study is to evaluate the ethanol extract of Rheum ribes root (RTE), as well as the root and young shoots (RYSE), for its chemical composition, antioxidant properties and cytotoxic effects. Total polyphenols (TPC), total flavonoid (TFC), radical scavenging activity (DPPH), the ferric reducing antioxidant power (FRAP) of Rheum ribes extracts were determined using colorimetric methods. Mineral contents and vitamin values of samples were determined by ICP-MS and HPLC, respectively. The cytotoxic effect of Rheum ribes extracts was determined on different cells using XTT assay. The cytotoxic effects of RTE and RYSE on cancer cells were evaluated with regard to apoptosis. According to results, mineral contents, vitamine A and C values were very high in both samples. Ethanolic extracts of Rheum ribes young shoot exhibited a selective cytotoxic effect on all cancer cells compared to WI-38 cells, and the IC50 values of the extract in the cancer cells between 26.10 to 54.81 μg/mL. Ethanolic extracts of RTE and RYSE induced apoptosis on MCF-7 cells. The ethanolic extracts of Rheum ribes has effective cytotoxic and antioxidant activity. More research is needed to determine the cytotoxic effect mechanisms on cancer cells.