一种定量测定铁离子螯合能力的分析方法:开发与验证

Q3 Agricultural and Biological Sciences Acta Scientiarum. Biological Sciences Pub Date : 2022-05-13 DOI:10.4025/actascibiolsci.v44i1.59739
Éverton da Silva Santos, L. Teixeira, J. C. Castro, Laura Paulino Mardigan, José Rivaldo dos Santos, J. Gonçalves, Arildo José Braz de Oliveira, R. A. C. Gonçalves
{"title":"一种定量测定铁离子螯合能力的分析方法:开发与验证","authors":"Éverton da Silva Santos, L. Teixeira, J. C. Castro, Laura Paulino Mardigan, José Rivaldo dos Santos, J. Gonçalves, Arildo José Braz de Oliveira, R. A. C. Gonçalves","doi":"10.4025/actascibiolsci.v44i1.59739","DOIUrl":null,"url":null,"abstract":"Iron is a fundamental microelement for human life; however, deficiencies or excesses of these metal ions can cause severe complications and mortality. Chelators are compounds that bind and inhibit iron. Ultraviolet-visible (UV-vis) spectrophotometric methods are key analytical tools in the identification of chemical entities, with the benefits of having good precision and accuracy, and the equipment being easily available as well as quick and simple to implement. In this study, we aimed to provide an alternative, cheaper method for the quantification of iron ion chelation by substituting ferrozine for gallic acid and validating its use with UV-vis according to official ANVISA and ICH guidelines. The parameters assessed were specificity, linearity, precision, accuracy, robustness, and finally, the percentage of iron ions chelating was calculated. The results demonstrated that this method was accurate, simple, specific, selective, precise, and reproducible, and was successfully validated for the determination of iron ions chelating. The percentage of iron ions chelating, promoted by the standard chelator EDTA, was 45% and 47% for Fe2+ and Fe3+, respectively. It is concluded that this new method is beneficial in terms of its simplicity, rapidness, low cost, and the fact that it produces very low levels of dangerous residues.","PeriodicalId":7166,"journal":{"name":"Acta Scientiarum. Biological Sciences","volume":"39 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2022-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":"{\"title\":\"An analytical method for the quantitative determination of iron ion chelating capacity: development and validation\",\"authors\":\"Éverton da Silva Santos, L. Teixeira, J. C. Castro, Laura Paulino Mardigan, José Rivaldo dos Santos, J. Gonçalves, Arildo José Braz de Oliveira, R. A. C. Gonçalves\",\"doi\":\"10.4025/actascibiolsci.v44i1.59739\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Iron is a fundamental microelement for human life; however, deficiencies or excesses of these metal ions can cause severe complications and mortality. Chelators are compounds that bind and inhibit iron. Ultraviolet-visible (UV-vis) spectrophotometric methods are key analytical tools in the identification of chemical entities, with the benefits of having good precision and accuracy, and the equipment being easily available as well as quick and simple to implement. In this study, we aimed to provide an alternative, cheaper method for the quantification of iron ion chelation by substituting ferrozine for gallic acid and validating its use with UV-vis according to official ANVISA and ICH guidelines. The parameters assessed were specificity, linearity, precision, accuracy, robustness, and finally, the percentage of iron ions chelating was calculated. The results demonstrated that this method was accurate, simple, specific, selective, precise, and reproducible, and was successfully validated for the determination of iron ions chelating. The percentage of iron ions chelating, promoted by the standard chelator EDTA, was 45% and 47% for Fe2+ and Fe3+, respectively. It is concluded that this new method is beneficial in terms of its simplicity, rapidness, low cost, and the fact that it produces very low levels of dangerous residues.\",\"PeriodicalId\":7166,\"journal\":{\"name\":\"Acta Scientiarum. Biological Sciences\",\"volume\":\"39 1\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2022-05-13\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Acta Scientiarum. Biological Sciences\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.4025/actascibiolsci.v44i1.59739\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"Agricultural and Biological Sciences\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Acta Scientiarum. Biological Sciences","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.4025/actascibiolsci.v44i1.59739","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"Agricultural and Biological Sciences","Score":null,"Total":0}
引用次数: 1

摘要

铁是人类生命的基本微量元素;然而,这些金属离子的缺乏或过量会引起严重的并发症和死亡。螯合剂是结合和抑制铁的化合物。紫外-可见(UV-vis)分光光度法具有精密度高、准确度高、设备方便、操作简便等优点,是化学实体鉴定的关键分析工具。在这项研究中,我们的目的是提供一种替代的,更便宜的方法来定量铁离子螯合作用,用亚铁锌代替没食子酸,并根据官方ANVISA和ICH指南验证其使用紫外线-可见。评价指标为特异性、线性、精密度、准确度、稳健性,最后计算铁离子螯合率。结果表明,该方法准确、简便、专属性强、选择性好、精密度高、重复性好,可用于铁离子螯合的测定。在标准螯合剂EDTA的促进下,铁离子对Fe2+和Fe3+的螯合率分别为45%和47%。结果表明,该方法具有简单、快速、成本低、有害残留物含量低等优点。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
An analytical method for the quantitative determination of iron ion chelating capacity: development and validation
Iron is a fundamental microelement for human life; however, deficiencies or excesses of these metal ions can cause severe complications and mortality. Chelators are compounds that bind and inhibit iron. Ultraviolet-visible (UV-vis) spectrophotometric methods are key analytical tools in the identification of chemical entities, with the benefits of having good precision and accuracy, and the equipment being easily available as well as quick and simple to implement. In this study, we aimed to provide an alternative, cheaper method for the quantification of iron ion chelation by substituting ferrozine for gallic acid and validating its use with UV-vis according to official ANVISA and ICH guidelines. The parameters assessed were specificity, linearity, precision, accuracy, robustness, and finally, the percentage of iron ions chelating was calculated. The results demonstrated that this method was accurate, simple, specific, selective, precise, and reproducible, and was successfully validated for the determination of iron ions chelating. The percentage of iron ions chelating, promoted by the standard chelator EDTA, was 45% and 47% for Fe2+ and Fe3+, respectively. It is concluded that this new method is beneficial in terms of its simplicity, rapidness, low cost, and the fact that it produces very low levels of dangerous residues.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Acta Scientiarum. Biological Sciences
Acta Scientiarum. Biological Sciences Agricultural and Biological Sciences-Agricultural and Biological Sciences (all)
CiteScore
1.00
自引率
0.00%
发文量
45
审稿时长
38 weeks
期刊介绍: The journal publishes original articles in all areas of Biological Sciences, including anatomy, bacteriology, molecular biology, biochemistry, botany, cytology and cell biology, animal behavior, ecology, limnology, embryology, and histology, morpho-physiology, genetics, microbiology, parasitology and zoology.
期刊最新文献
Characterization of the digestive microbiome of invasive Limnoperna fortunei (Dunker, 1857) collected in different seasons in the upper Uruguay River, Santa Catarina, Brazil Genetic diversity among genotypes of Parkia platycephala (Benth.), a typical tree of northeastern Brazil Bat fauna from the Sooretama Biological Reserve, southeastern Brazil A comparative investigation of bioactive compounds present in Datura stramonium L. leaves extract in various solvents (ethanol, aqua, and ether) using TLC, IR, and GC-MS analytical techniques Parasites of juvenile Salminus brasiliensis and Brycon orbignyanus (Characiformes, Bryconidae) in the middle Paraná River, Argentina
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1