五角草(Ceiba pentandra)遗传变异分析Gaertn。利用RAPD分子标记对西苏门答腊几个关键土地的研究

Fadilla Hefzi, M. Mansyurdin, T. Maideliza
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引用次数: 0

摘要

五角草(L.)Gaertn。是一种果实可用于生产纤维和生物燃料种子的植物,它具有在关键土地上开发的潜力,因为它适应在贫瘠缺水的关键土地上生长。在西苏门答腊,几个关键的土地上长满了五角草。本研究旨在确定西苏门答腊岛5个关键陆地种群中五角草的遗传变异,为在关键土地上选择优质种子提供依据。本研究采用描述方法,利用分子标记RAPD(随机扩增多态性DNA)进行分子数据分析。结果表明,引物OPA-01、OPA-02和OPB-10能够检测到多态性。利马Puluh Kota县的Pangkalan Koto Baru (H = 0.1212)是种群内遗传变异值最高的种群。种群间遗传变异(DST = 0.0321)低于种群内遗传变异(HS = 0.1021),遗传分化值低(GST = 0.2392),基因流动值高(Nm = 1.5894)。五角草的遗传变异可用于植物育种中的亲本选择。
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Analysis of Genetic Variations of Ceiba pentandra (L.) Gaertn. on Several Critical Lands in West Sumatra Using RAPD Molecular Markers
Ceiba pentandra (L.) Gaertn. is a plant whose fruit is used to produce fiber and seeds for biofuel, which has the potential to be developed in critical land because it is adaptive to grow in critical land that is less fertile and lacks water. In West Sumatra, several critical land locations are overgrown by C. pentandra plants. This study aims to determine the genetic variation of C. pentandra in five critical land populations in West Sumatra as the basis for selecting superior seeds for development in critical land. The research was conducted by the descriptive method using molecular data with the molecular marker RAPD (random amplified polymorphic DNA). The results showed that the primers OPA-01, OPA-02, and OPB-10 could detect polymorphisms. Pangkalan Koto Baru in the regency Lima Puluh Kota (H = 0.1212) was the population with the highest intrapopulation genetic variation value. Interpopulation genetic variation (DST = 0.0321) was lower than intrapopulation genetic variation (HS = 0.1021), with a low genetic differentiation value (GST = 0.2392) and a high gene flow value (Nm = 1.5894). The genetic variation of C. pentandra can be used to select the parent in plant breeding programs.
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CiteScore
1.20
自引率
0.00%
发文量
13
审稿时长
16 weeks
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