{"title":"作为潜在毒性标志的大肠杆菌过氧化水平:镍和甲基对硫磷的影响","authors":"M. Espigares, L. Lucadamo, C. Coca, E. Aloj, R. Gálvez","doi":"10.1002/TOX.2530090307","DOIUrl":null,"url":null,"abstract":"The effects of nickel and methyl-parathion were studied in subinhibiting concentrations (40 and 62.5 μg/mL, respectively) on the levels of cellular peroxidation in Escherichia coli. These effects were evaluated using the thiobarbituric acid (TBA) test and the determination of lipofuscin-like substances. Both toxicants caused an increase in cellular peroxidation levels, although at the concentrations used, nickel was shown to be a better inducer than methyl-parathion. The TBA test gave more significant differences than the determination of lipofuscin-like substances when the control cultures were compared to those containing the toxicant. This finding reveals that the TBA test could be used as a toxicity marker in E. coli for the development of ecotoxicity biotests. © 1994 by John Wiley & Sons, Inc..","PeriodicalId":11824,"journal":{"name":"Environmental Toxicology & Water Quality","volume":"1 1","pages":"203-208"},"PeriodicalIF":0.0000,"publicationDate":"1994-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"4","resultStr":"{\"title\":\"Peroxidation levels in Eschericia coli as a potential toxicity marker: Effect of nickel and methyl-parathion\",\"authors\":\"M. Espigares, L. Lucadamo, C. Coca, E. Aloj, R. Gálvez\",\"doi\":\"10.1002/TOX.2530090307\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"The effects of nickel and methyl-parathion were studied in subinhibiting concentrations (40 and 62.5 μg/mL, respectively) on the levels of cellular peroxidation in Escherichia coli. These effects were evaluated using the thiobarbituric acid (TBA) test and the determination of lipofuscin-like substances. Both toxicants caused an increase in cellular peroxidation levels, although at the concentrations used, nickel was shown to be a better inducer than methyl-parathion. The TBA test gave more significant differences than the determination of lipofuscin-like substances when the control cultures were compared to those containing the toxicant. This finding reveals that the TBA test could be used as a toxicity marker in E. coli for the development of ecotoxicity biotests. © 1994 by John Wiley & Sons, Inc..\",\"PeriodicalId\":11824,\"journal\":{\"name\":\"Environmental Toxicology & Water Quality\",\"volume\":\"1 1\",\"pages\":\"203-208\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1994-08-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"4\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Environmental Toxicology & Water Quality\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1002/TOX.2530090307\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Environmental Toxicology & Water Quality","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1002/TOX.2530090307","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 4
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