大鼠肾皮质细胞胞浆中金的表征

Howard O. Thompson, Judith Blaszak, Connie J. Knudtson , C.Frank Shaw III
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引用次数: 29

摘要

证据表明,在大鼠肾皮质细胞细胞质中巯基结合位点之间存在金(I)的化学平衡。通过凝胶渗透色谱法,在体内给药硫硫酸金钠、硫葡萄糖金钠、NaAuCl4和(C2H5)3PAuCl后,蛋白质和非蛋白质物种中的金分布是相似的,尽管细胞质中的金浓度有所不同。金的洗脱模式与上清液中硫醇基团的模式非常相似。在离体上清馏分中加入硫硫酸金钠产生了相同的分布。在体内给金后,在细胞质中加入半胱氨酸,使蛋白结合金急剧减少,而非蛋白结合金急剧增加。这些数据排除了诱导合成金属硫蛋白样金结合蛋白的可能性。超离心显示金存在于微粒体部分和上清中。
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Characterization of gold in the cytosol of rat-kidney cortex cells

Evidence is presented for a chemical equilibrium of gold(I) among sulfhydryl binding sites in the cytoplasm of renal cortical cells of the rat. The gold distribution among protein and nonprotein species, examined by gel-permeation chromatography following in vivo administration of gold sodium thiomalate, gold sodium thioglucose, NaAuCl4, and (C2H5)3PAuCl, was similar in each case, although the cytoplasmic gold concentrations varied. The pattern of gold elution closely parallels that of the thiol groups in the supernatant. In vitro addition of gold sodium thiomalate to the supernatant fraction produced an identical distribution. Addition of cysteine to cytoplasm following in vivo administration of gold drastically decreased the protein-bound gold and increased the non-protein-bound gold. These data rule out the induced synthesis of a metallothionein-like gold-binding protein. Ultracentrifugation revealed gold to be present in the microsomal fraction as well as the supernatant.

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