棕榈压榨纤维、空果束和棕榈干中的纤维素分解真菌

D. C. Onyia, E. Onyeneke, T. Okunwaye, E. Okogbenin, B. O. Okogbenin, E. Idabie, N. U. Asiriuwa, H. Anemene
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摘要

纤维素分解真菌在纸浆和造纸、农业、生物燃料生产和纺织等各个行业都显示出潜在的应用前景。本研究的目的是对棕榈中纤维素分解真菌进行分离、筛选和鉴定。分离筛选了20株真菌。7株分离菌株:3株曲霉、2株木霉、1株根霉和1株青霉具有显著的纤维素水解活性。木霉品种C(来自棕榈压榨纤维)的清除率最高,为11.5mm。根霉种(A)和木霉种(B)(分别来自空果束和棕榈压榨纤维)的清除率分别为8.5mm和6.5mm,曲霉种(D和E)(棕榈果束和空果束)的清除率最小,均为1.0mm,持续时间为3-5 D。到第4天时,所有菌株的菌丝质量均较高,其中木霉(C)菌丝质量最高;其次是木霉(B) (0.58g)和曲霉(D) (0.53g)。曲霉(E)菌丝质量最小(0.38g)。经分离筛选的木霉、根霉和曲霉可能是纤维素酶的潜在来源。
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Cellulolytic Fungi from Palm Pressed Fiber, Empty Fruit Bunch, and Palm Trunk
Cellulolytic fungi have shown potential applications in various industries such as pulp and paper, agriculture, biofuel production, and textile. The purpose of this is study to isolate, screen and identify cellulolytic fungi from palm. Twenty (20) fungi strains were isolated and screened. Seven (7) isolates: 3 Aspergillus spp, (2) Trichoderma spp, 1 Rhizopus species, and 1 Penicillium spp had significant cellulolytic activity. Trichoderma species, C (from palm pressed fiber), showed the highest clearance zone of 11.5mm. Also, Rhizopus species (A) and Trichoderma species (B) (from empty fruit bunch and palm pressed fiber, respectively), showed high clearance zone of 8.5mm, and 6.5mm, respectively, with Aspergillus species (D and E) (palm truck and empty fruit bunch), having the most minor clearance zone of 1.0mm, each for the 3-5 days assayed. All the fungi isolates elicited high mycelia weight by the 4th day, with Trichoderma species(C) having the highest; 0.79g, followed by Trichoderma species (B) (0.58g) and Aspergillus species (D) (0.53g). Aspergillus species (E) (0.38g) showed the least mycelia weight. Trichoderma species, Rhizopus species, and Aspergillus species isolated and screened could be potential sources of cellulases for industrial application.
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