Traditional Treatment by Arabic Acacia Seeds Exterminating Microbial Biofilms for Wounds Healing

Microbial biofilms exist in wounds resulting in long-term non-healing infected wounds. Herbal products as ancient traditions treatment and scientists in developing countries using in wounds healing over years. Arabic Acacia present in KSA, it's using in production of some medications. It has gallic-acid, methyl-gallate, quercetin, rutin, myricetin 3-O-(3'''-O -galloyl), β-D-rutinoside and catechin. It has a significant antimicrobial agent. Its extract provided fast wounds healing related to antimicrobial activities. The aim was to prove "Traditional Treatment" by Arabic Acacia Seeds exterminate microbial biofilms for wounds healing. Materials and methods were included Arabic Acacia aqueous extract, biofilm isolates included (Staphylococcus sp., Streptococcus sp., Pseudomonas sp., Proteus sp., Escherichia coli, also Klebsiella sp., and Candida sp.)) The applied tests were anti-biofilms formation and biofilms elimination, then biofilm estimation by Tissue Culture Plate method (TCP). Results were for estimation of anti-biofilms formation after exposed to Arabic Acacia aqueous extract. It was eliminated by simple Arabic Acacia aqueous extract early by crude dilution in overnight time were Streptococcus sp., and Proteus sp. Then by crude dilution in day time were Escherichia coli, also Klebsiella sp. Staphylococcus sp., Pseudomonas sp., and Candida sp., they had reached the weak point only. Estimation of biofilms elimination after exposed to Arabic Acacia aqueous extract. The effects of all dilutions were faster than anti-biofilms formation. This was giving none for Streptococcus sp., and Proteus sp., by half dilution for overnight. While Streptococcus sp., Proteus sp., Escherichia coli also Klebsiella sp. were resulted none by half dilution for a day. Crude dilution gave none for all biofilms elimination in both times. It was concluded that the Arabic Acacia aqueous extract had microbial anti-biofilms formation. That was by their antimicrobial contents exterminating microbial biofilms for wounds healing. This indicated the using from ancient Arab customs to treat wounds as traditional treatment. It was recommended that for the "Traditional Treatment" of Arabic Acacia extract according healthy doses as simple treatment instead of chemical treatment for wounds healing.