J. Quiroz, Cristian Lizarazo, Jesús García, J. Torres, Israel García, Jose Hernández
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引用次数: 0
摘要
浆果是植物营养素的丰富来源,尤其是类黄酮等酚类化合物,具有抗氧化特性。在这些水果中,种植和消费最多的是Fragaria属(草莓)和Rubus属(覆盆子、黑莓、露莓),它们对人类和动物健康的有益影响已被广泛研究。这些水果中最重要的生物活性化合物之一是花青素,它具有抗菌、抗炎和抗癌活性,对健康有潜在的好处。因此,花青素的研究具有重要的医药和营养价值。本研究的目的是分析产自墨西哥Michoacán州的腺毛楸(Rubus adenotrichos)和花青素(Fragaria x ananassa)的生物动力学机制。为此,研究策略包括花青素的提取和定量,以及生物信息学工具来了解其在上述水果中的生物合成途径。利用信息学平台鉴定了参与腺毛藓和黄花楸中花青素生物合成的调控基因和酶,发现大多数基因和酶是共同的,有一些特定的差异,只有少数例外,如儿茶酚- o -甲基转移酶(OMT)、udp -葡萄糖基转移酶(UGT)和β -葡萄糖醛酸酶(GUSB)酶,这些酶只出现在腺毛藓中,而不出现在黄花楸中。
Biokinetic mechnisms of anthocyanins in red fruits produced in the state of Michoacan, Mexico
Berry fruits are a rich source of phytonutrients, especially phenolic compounds such as flavonoids, which have antioxidant properties. Among these fruits, the most cultivated and consumed are those of the genus Fragaria (Strawberries) and Rubus (Raspberries, blackberries, dewberries), which have been widely studied for their beneficial effects on human and animal health. One of the most important bioactive compounds of these fruits are anthocyanins, which have shown potential benefits for health by their antimicrobial, anti-inflammatory and anticancer activity. Therefore, the study of anthocyanins is of great pharmaceutical and nutraceutical interest. The objective of this research is to analyze the biokinetic mechanisms of anthocyanins in Rubus adenotrichos and Fragaria x ananassa produced in the state of Michoacán, Mexico. For this purpose, research strategies that included the extraction and quantification of anthocyanins, as well as bioinformatic tools to understand their biosynthetic pathway in the mentioned fruits were used. The use of informatic platforms allowed to identify the regulatory genes and enzymes involved in the biosynthesis of anthocyanins in R. adenotrichos and F. x ananassa, finding that most are common, with some specific differences, and that there are only a few exceptions, such as the enzymes catechol-O-methyltransferase (OMT), UDP-glucosyltransferase (UGT) and beta-glucuronidase (GUSB), which only occur in Rubus adenotrichos and not in Fragaria x ananassa.
期刊介绍:
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